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Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection
期刊论文
TALANTA, 2023, 卷号: 252, 页码: 7
作者:
Guo, Zixuan
;
Tan, Xiao
;
Yuan, Haoyu
;
Zhang, Ling
;
Wu, Jiajia
收藏
  |  
浏览/下载:17/0
  |  
提交时间:2023/01/06
MicroRNA
CRISPR-Cas12a
T7 RNA polymerase
All -in -one
T7 phage factor required for managing RpoS in Escherichia coli
期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 卷号: 115, 页码: E5353-E5362
作者:
Tabib-Salazar, Aline
;
Liu, Bing
;
Barker, Declan
;
Burchell, Lynn
;
Qimron, Udi
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  |  
浏览/下载:7/0
  |  
提交时间:2019/11/26
transcription regulation
T7 phage
Escherichia coli
RNA polymerase
stationary phase
Molecular dynamics simulation study of the "stay or leave" problem for two magnesium ions in gene transcription
期刊论文
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2017, 卷号: 85, 期号: 6, 页码: 1002-1007
作者:
Wu, SG
;
Wu, SG (reprint author), Sichuan Normal Univ, Coll Chem & Mat Sci, Chengdu 610066, Peoples R China.
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  |  
浏览/下载:20/0
  |  
提交时间:2017/12/21
Molecular Dynamics Simulation
T7 Rna Polymerase
Magnesium Ion
Transcription Mechanism
Free Energy
Mechanism of NTP Binding to the Active Site of T7 RNA Polymerase Revealed by Free-Energy Simulation
期刊论文
BIOPHYSICAL JOURNAL, 2017, 卷号: 112, 期号: 11, 页码: 2253-2260
作者:
Wu, SG
;
Li, LC
;
Li, Q
;
Wu, SG (reprint author), Sichuan Normal Univ, Coll Chem & Mat Sci, Chengdu, Peoples R China.
;
Wu, SG (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing, Peoples R China.
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  |  
浏览/下载:20/0
  |  
提交时间:2017/12/21
Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method
期刊论文
Chemical Communications, 2017, 卷号: 53, 期号: 43, 页码: 5846-5849
作者:
Zhang, Kai*
;
Wang, Ke
;
Zhu, Xue
;
Xie, Minhao*
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  |  
浏览/下载:2/0
  |  
提交时间:2019/12/04
T7 RNA polymerase translocation is facilitated by a helix opening on the fingers domain that may also prevent backtracking
期刊论文
NUCLEIC ACIDS RESEARCH, 2017
Da, Lin-Tai
;
E, Chao
;
Shuai, Yao
;
Wu, Shaogui
;
Su, Xiao-Dong
;
Yu, Jin
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  |  
浏览/下载:5/0
  |  
提交时间:2017/12/03
MARKOV STATE MODELS
MOLECULAR-DYNAMICS
STRUCTURAL BASIS
CONFORMATIONAL DYNAMICS
ELONGATION COMPLEXES
GENOME INSTABILITY
TRANSCRIPTION
TRANSITION
SIMULATIONS
INITIATION
Novel T7-like expression systems used for Halomonas
期刊论文
METABOLIC ENGINEERING, 2017
Zhao, Han
;
Zhang, Haoqian M.
;
Chen, Xiangbin
;
Li, Teng
;
Wu, Qiong
;
Ouyang, Qi
;
Chen, Guo-Qiang
收藏
  |  
浏览/下载:12/0
  |  
提交时间:2017/12/03
Part mining
Expression system
Morphological engineering
PHB
Synthetic biology
Non-model microorganism
BACTERIOPHAGE-T7 RNA-POLYMERASE
EUROPEAN VECTOR ARCHITECTURE
GENETIC CIRCUIT-DESIGN
ESCHERICHIA-COLI
SYNTHETIC BIOLOGY
ENVIRONMENTAL SIGNAL
MAMMALIAN-CELLS
PROMOTERS
SEQUENCE
ACID
The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase
期刊论文
RNA BIOLOGY, 2017, 卷号: 14, 期号: 10, 页码: 1314-1319
作者:
Gong, Peng
;
Shu, Bo
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  |  
浏览/下载:21/0
  |  
提交时间:2018/12/29
Active site closure
crystal structure
nucleotide addition cycle
RNA-dependent
RNA polymerase
translocation
Mechanism of pyrophosphate ion release in T7 RNA polymerase revealed by free energy simulations
期刊论文
COMPUTATIONAL AND THEORETICAL CHEMISTRY, 2016, 卷号: 1094, 页码: 127-132
作者:
Wu, SG
;
Jiang, MQ
;
Liu, L
;
Wu, SG (reprint author), Sichuan Normal Univ, Coll Chem & Mat Sci, Chengdu 610068, Peoples R China.
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  |  
浏览/下载:17/0
  |  
提交时间:2017/10/13
Molecular Dynamics Simulation
T7 Rna Polymerase
Ppi Release
Potential Of Mean Force
Mechanism of pyrophosphate ion release in T7 RNA polymerase revealed by free energy simulations
期刊论文
COMPUTATIONAL AND THEORETICAL CHEMISTRY, 2016, 卷号: 1094, 页码: 127-132
作者:
Wu, SG
;
Jiang, MQ
;
Liu, L
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  |  
浏览/下载:109/0
  |  
提交时间:2019/04/08
Molecular dynamics simulation
PARTICLE MESH EWALD
T7 RNA polymerase
MOLECULAR-DYNAMICS
PPi release
TRANSLOCATION
Potential of mean force
DNA
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