Structural mechanism of the active bicarbonate transporter from cyanobacteria

doi:10.1038/s41477-019-0538-1

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Structural mechanism of the active bicarbonate transporter from cyanobacteria
	Wang, CC ; Sun, B ; Zhang, X ; Huang, XW ; Zhang, MH ; Guo, H ; Chen, X ; Huang, F ; Chen, TY ; Mi, HL
刊名	NATURE PLANTS
	2019
卷号	5 期号:11 页码:1184-+
关键词	RENAL TUBULAR-ACIDOSIS STAS DOMAIN FUNCTIONAL-CHARACTERIZATION INCREASE PHOTOSYNTHESIS SULFATE TRANSPORTER ANION TRANSPORTERS CRYSTAL-STRUCTURE MUTATIONS MEMBRANE FAMILY
ISSN号	2055-026X
DOI	10.1038/s41477-019-0538-1
文献子类	期刊论文
英文摘要	Bicarbonate transporters play essential roles in pH homeostasis in mammals and photosynthesis in aquatic photoautotrophs. A number of bicarbonate transporters have been characterized, among which is BicA-a low-affinity, high-flux SLC26-family bicarbonate transporter involved in cyanobacterial CO2-concentrating mechanisms (CCMs) that accumulate CO2 and improve photosynthetic carbon fixation. Here, we report the three-dimensional structure of BicA from Synechocystis sp. PCC6803. Crystal structures of the transmembrane domain (BicA (TM)) and the cytoplasmic STAS domain (BicA(STAS)) of BicA were solved. BicA (TM) was captured in an inward-facing HCO3--bound conformation and adopts a '7+7' fold monomer. HCO3- binds to a cytoplasm-facing hydrophilic pocket within the membrane. BicA(STAS) is assembled as a compact homodimer structure and is required for the dimerization of BicA. The dimeric structure of BicA was further analysed using cryo-electron microscopy and physiological analysis of the full-length BicA, and may represent the physiological unit of SLC26-family transporters. Comparing the BicA (TM) structure with the outward-facing transmembrane domain structures of other bicarbonate transporters suggests an elevator transport mechanism that is applicable to the SLC26/4 family of sodium-dependent bicarbonate transporters. This study advances our knowledge of the structures and functions of cyanobacterial bicarbonate transporters, and will inform strategies for bioengineering functional BicA in heterologous organisms to increase assimilation of CO2.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32220]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Natl Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China; 4.Univ Liverpool, Inst Integrat Biol, Liverpool, Merseyside, England; 5.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, CC,Sun, B,Zhang, X,et al. Structural mechanism of the active bicarbonate transporter from cyanobacteria[J]. NATURE PLANTS,2019,5(11):1184-+.
APA	Wang, CC.,Sun, B.,Zhang, X.,Huang, XW.,Zhang, MH.,...&Zhang, P.(2019).Structural mechanism of the active bicarbonate transporter from cyanobacteria.NATURE PLANTS,5(11),1184-+.
MLA	Wang, CC,et al."Structural mechanism of the active bicarbonate transporter from cyanobacteria".NATURE PLANTS 5.11(2019):1184-+.