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沈阳应用生态研究所 [3]
武汉病毒研究所 [2]
自然资源部第一海洋研... [2]
北京大学 [1]
西安交通大学 [1]
大连理工大学 [1]
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期刊论文 [18]
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2019 [3]
2017 [5]
2016 [1]
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2009 [1]
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Production of "biobetter" variants of glucarpidase with enhanced enzyme activity
期刊论文
BIOMEDICINE & PHARMACOTHERAPY, 2019, 卷号: 112
作者:
Al-Qahtani, Alanod D.
;
Bashraheel, Sara S.
;
Rashidi, Fatma B.
;
O'Connor, C. David
;
Romero, Atilio Reyes
收藏
  |  
浏览/下载:61/0
  |  
提交时间:2019/11/19
Glucarpidase
Biobetter glucarpidase
DNA shuffling
Drug detoxification
ADEPT
Error-prone PCR
Targeted cancer therapy
Modular Engineering of the Flavin Pathway in Escherichia coli for Improved Flavin Mononucleotide and Flavin Adenine Dinucleotide Production
期刊论文
Journal of Agricultural and Food Chemistry, 2019, 卷号: Vol.67 No.23, 页码: 6532-6540
作者:
Liu, S.
;
Diao, N.
;
Wang, Z.
;
Lu, W.
;
Tang, Y.-J.
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  |  
浏览/下载:2/0
  |  
提交时间:2019/11/21
error-prone PCR
Escherichia coli
flavins
modular engineering
Modular Engineering of the Flavin Pathway in Escherichia coli for Improved Flavin Mononucleotide and Flavin Adenine Dinucleotide Production
期刊论文
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 卷号: 67, 期号: 23, 页码: 6532-6540
作者:
Liu, Shuang
;
Diao, Na
;
Wang, Zhiwen
;
Lu, Wenyu
;
Tang, Ya-Jie
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  |  
浏览/下载:6/0
  |  
提交时间:2019/12/11
flavins
Escherichia coli
modular engineering
error-prone PCR
Characterization of a metagenome-derived protease from contaminated agricultural soil microorganisms and its random mutagenesis
期刊论文
FOLIA MICROBIOLOGICA, 2017, 卷号: 62, 期号: 6, 页码: 499-508
作者:
Jiang, Chengjian
;
Zhang, Liang
;
Li, Fajia
;
Meng, Can
;
Zeng, Rong
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  |  
浏览/下载:6/0
  |  
提交时间:2018/12/07
Metagenome-derived protease
Soil microorganisms
Biochemical characterization
Error-prone PCR
Characterization of a metagenome-derived protease from contaminated agricultural soil microorganisms and its random mutagenesis
期刊论文
FOLIA MICROBIOLOGICA, 2017, 卷号: 62, 期号: 6, 页码: 499-508
作者:
Jiang, Chengjian
;
Zhang, Liang
;
Li, Fajia
;
Meng, Can
;
Zeng, Rong
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  |  
浏览/下载:3/0
  |  
提交时间:2019/08/19
Metagenome-derived protease
Soil microorganisms
Biochemical characterization
Error-prone PCR
Engineering of Harobin for enhanced fibrinolytic activity obtained by random and site-directed mutagenesis
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2017
Li, Zhuojian
;
Chen, Xiaojia
;
Guo, Shujun
;
Zhang, Huihua
;
Dong, Haojun
;
Wu, Guoyi
;
Hong, An
;
Gu, Jun
收藏
  |  
浏览/下载:107/0
  |  
提交时间:2017/12/03
Random mutagenesis
Double mutant
Harobin
Thrombosis
Hypertension
ERROR-PRONE PCR
SNAKE-VENOM
HYPERTENSIVE-RAT
PLATELET-AGGREGATION
ORAL ANTICOAGULANTS
PICHIA-PASTORIS
EVOLUTION
HEMOSTASIS
PROTEINS
IDENTIFICATION
Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR)
期刊论文
Methods in molecular biology (Clifton, N.J.), 2017, 卷号: 1498, 页码: 497-506
作者:
Shao Weilan[1]
;
Ma Kesen[2]
;
Le Yilin[3]
;
Wang Hongcheng[4]
;
Sha Chong[5]
收藏
  |  
浏览/下载:3/0
  |  
提交时间:2019/12/24
Amplification of circular plasmids
Directed evolution
In situ error-prone PCR
Random mutagenesis
Selection marker swapping
Thermostable DNA ligase
Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1-2 by Directed Evolution
期刊论文
2017, 卷号: 22, 期号: 12
作者:
Chen, Jing-Jing
;
Liang, Xiao
;
Li, Hui-Xian
;
Chen, Tian-Jiao
;
Zhu, Ping
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2020/01/03
glycoside hydrolase
directed evolution
error-prone PCR
catalytic efficiency
7--Xylosyltaxanes
Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp SN5 by random mutation and Glu135 saturation mutagenesis
期刊论文
BMC BIOTECHNOLOGY, 2016, 卷号: 16
作者:
Bai, Wenqin
;
Cao, Yufan
;
Liu, Jun
;
Wang, Qinhong
;
Jia, Zhenhu
收藏
  |  
浏览/下载:31/0
  |  
提交时间:2017/03/13
Xylanase
Alkalophilicity
Error-prone PCR
Site-directed mutation
Site-saturation mutagenesis
Catalytic efficiency
Directed evolution of the CpcA biosynthetic pathway and optimization of conditions for CpcA production and its properties
期刊论文
2014
作者:
于平[1]
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  |  
浏览/下载:2/0
  |  
提交时间:2019/12/26
CpcA
Directed evolution
Error-prone PCR
Spectrum
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