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浏览/检索结果: 共1条，第1-1条 帮助 限定条件 专题：中南林业科技大学    第一署名单位    第一作者单位    通讯作者单位     已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 Specific and Rapid Detection of Camellia oleifera Anthracnose Pathogen by Nested-PCR 期刊论文 African Journal of Biotechnology, 2009, 卷号: 8, 期号: 6, 页码: 1056-1061 作者:  Liu, Junang;  He, Li*;  Zhou, Guoying 收藏  |  浏览/下载：4/0  |  提交时间：2019/12/28 Camellia oleifera is an economical important plant in southern China for edible oil production. Anthracnose is a serious disease that limited its development. The internal transcribed spacer (ITS) regions and the 5.8S rRNA gene of strain C1 of the pathogenic fungus Colletetrichum gloeosporioides were sequenced in order to design specific PCR primers for pathogen detection. Alignment of the sequence data of strain C1 and the other Colletetrichum species obtained from the Genbank were made using CLUSTAL W. Based on the aligned ITS sequences  specific primers for C. gloeosporioides were developed (YT1 and YT2). The infecting pathogens were successfully detected with our specific primer set and showed high specificity. The result showed that the nested-PCR reaction was at least 10  000-fold more specific than that of the simple PCR method. This new method provides a useful technique to further study disease cycle and for early prediction of anthracnose of Camellia oleifera.