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浏览/检索结果: 共1条，第1-1条 帮助 限定条件 专题：中南林业科技大学    第一署名单位    第一作者单位    通讯作者单位     已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 Screening and fermentation optimization of microbial lipid-producing molds from forest soils 期刊论文 AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2010, 卷号: 4, 期号: 14, 页码: 1462-1468 作者:  Liu, Gao-Qiang*;  Lin, Qin-Lu;  Jin, Xian-Chun;  Wang, Xiao-Ling;  Zhao, Yan 收藏  |  浏览/下载：3/0  |  提交时间：2019/12/28 Sixty-one isolates including molds  yeasts and bacteria from the forest soils collected from Hunan Province  China were tested for their potentiality to accumulate microbial lipid as alternative sources for biodiesel production. The results showed that sixteen mold isolates were potential oleaginous microorganisms  among which  strain SCIM 3.009 was the best lipid producers  which may accumulate up to 50.4% of lipids in dry biomass. Further study showed that the components profile of the lipid from strain SCIM 3.009 had the similar characters to that of vegetable oil  abundant in low degree unsaturated long chain fatty acid (C18:1) and saturated long chain fatty acids (C16:0)  suggesting the lipid is a potential source for biodiesel production. Based on the morphology and a commercial identification system  the strain SCIM 3.009 was found to be Thamnidium ctenidium. To enhance the lipid production by the strain  the fermentation parameters were optimized  the optimium conditions were as follows: glucose as carbon source with initial concentration 60 g/L  NH4NO3 as nitrogen source at 3.0 g/L  culture temperature was 30°C  initial pH = 6.5  culture volume was 50 mL in a 250 mL flask  agitation speed was 220 rpm. Results on verification of the optimium conditions in a 5 L stirred-tank bioreactor reveal that the strain accumulate up to 66.02% of lipids in dry biomass and the lipid yield significantly enhanced from 6.4 ± 0.39 to 13.6 ± 0.37 g/L  while biomass enhanced from 12.7 ± 0.72 to 20.6 ± 0.52 g/L.