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浏览/检索结果: 共2条，第1-2条 帮助 已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE) 会议论文 International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009, Beijing, China Wang Y.-L.; Song K.-F.; Zhang W.-L.; Li J.-L. 收藏  |  浏览/下载：24/0  |  提交时间：2013/03/25 In the device  a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source  and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA) [1]. The first one is to design optimum exciting optical system  so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least  the sample fluorescence through the integrating sphere and come to the grating monochromator  The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device  SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process  signal-to-noise ratio(SNR) will be improved  also adjust delay time  ampling frequency and sampling number arbitrarily. By testing  the sensitivity is 10-12mol/L(substance marked by Eu3+)  examination repeat is &le2.5%  examination linearity is from 10 -8mol/L to 10-12mol/L  correlation coefficient is 99.98%(p&le0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody  and solve the tumor  genetic variation  the virus protein detection. 2009 SPIE.   Development and clinical research of the He-Ne laser medical instrument with quasi-single beam in two ways 会议论文 Conference on Lasers in Medicine and Dentistry - Diagnostics and Treatment, BEIJING, PEOPLES R CHINA 作者:  Li, ZH;  Zhang, Z;  Chen, PS 收藏  |  浏览/下载：2/0  |  提交时间：2020/01/06 He-Ne laser instrument  near-sightedness  radiation