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大连理工大学 [1]
长春光学精密机械与物... [1]
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会议论文 [2]
发表日期
2009 [1]
1996 [1]
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A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE)
会议论文
International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009, Beijing, China
Wang Y.-L.
;
Song K.-F.
;
Zhang W.-L.
;
Li J.-L.
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浏览/下载:24/0
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提交时间:2013/03/25
In the device
a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source
and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA) [1]. The first one is to design optimum exciting optical system
so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least
the sample fluorescence through the integrating sphere and come to the grating monochromator
The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device
SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process
signal-to-noise ratio(SNR) will be improved
also adjust delay time
ampling frequency and sampling number arbitrarily. By testing
the sensitivity is 10-12mol/L(substance marked by Eu3+)
examination repeat is &le2.5%
examination linearity is from 10 -8mol/L to 10-12mol/L
correlation coefficient is 99.98%(p&le0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody
and solve the tumor
genetic variation
the virus protein detection. 2009 SPIE.
Development and clinical research of the He-Ne laser medical instrument with quasi-single beam in two ways
会议论文
Conference on Lasers in Medicine and Dentistry - Diagnostics and Treatment, BEIJING, PEOPLES R CHINA
作者:
Li, ZH
;
Zhang, Z
;
Chen, PS
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浏览/下载:2/0
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提交时间:2020/01/06
He-Ne laser instrument
near-sightedness
radiation
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