A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia

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	A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia
	Wang, Xiaofei 1,2,3; Liu, Baozhong1 ; Liu, Fengsong 2; Huan, Pin 1
刊名	ZOOLOGICAL SCIENCE
	2015-10-01
卷号	32 期号:5 页码:419-426
关键词	bivalve calaxin cilia development larva
英文摘要	calaxin is a newly identified calcium sensor gene that modulates the movement of flagella (and possibly cilia). It was first identified from the ascidian Ciona intestinalis, and its orthologs have been observed in a wide range of animals and choanoflagellates. However, no calaxin-ortholog in a Lophotrochozoa species has been reported so far. This leaves open the question of whether the modulation of ciliary motility by calaxin is conserved among animals. We report a calaxin gene from the Pacific oyster Crassostrea gigas. This gene, termed cgi-calaxin, possesses three conserved EF-hand motifs as its orthologs from chordates. A phylogenetic analysis confirmed its orthology. Expression analysis revealed high expression in typical ciliated tissues such as gill and hepatopancreas. The spatiotemporal expression of the gene during early development was investigated using whole mount in situ hybridization. The results revealed that cgi-calaxin mRNA was aggregated in ciliated tissues of early larvae such as prototroch and velum. Immunofluorescence experiment further certified the ciliary localization of Cgi-Calaxin protein in D-veligers. We prepared the recombinant protein of cgi-calaxin and proved it had the capacity to bind to calcium. These results support the conserved roles of cgi-calaxin as a calcium sensor in ciliated cells and enrich the current knowledge on regulatory mechanisms of molluscan cilia. Moreover, we found the gene to be expressed in poorly ciliated tissues, such as adductor muscle, indicating possible roles in non-ciliated cells, which merits further investigation.
收录类别	SCI
语种	英语
WOS记录号	WOS:000361955400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/50936]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Hebei Univ, Baoding, Peoples R China 3.Chinese Peoples Liberat Army Gen Hosp, Dept Clin Lab, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaofei,Liu, Baozhong,Liu, Fengsong,et al. A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia[J]. ZOOLOGICAL SCIENCE,2015,32(5):419-426.
APA	Wang, Xiaofei,Liu, Baozhong,Liu, Fengsong,&Huan, Pin.(2015).A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia.ZOOLOGICAL SCIENCE,32(5),419-426.
MLA	Wang, Xiaofei,et al."A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia".ZOOLOGICAL SCIENCE 32.5(2015):419-426.