Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation
	Zhang, Zhendong 1; Mo, Zhaolan 2; Chen, Shiyong 2; Zou, YuXia 2; Zhang, Peijun 2
刊名	JOURNAL OF ENVIRONMENTAL BIOLOGY
	2015-07-01
卷号	36 期号:2015 页码:807-811
关键词	angR gene Iron uptake system Insertional inactivation Vibrio anguillarum
英文摘要	Vibrio anguillarum strain M3 was isolated from cultured diseased flounder Paralichthys olivaceus. In order to determine whether chromosomal angR gene plays an important role in infecting flounder host, part fragment of a virulence regulatory gene angR from V anguillarum M3 genome was cloned and ligated with suicide plasmids pNQ705 in the present study. The conserved fragment of angR gene was amplified from M3 genome and inserted into suicide plasmid pNQ705, a kind of conditional replicon. The recombinant plasmid was transferred into Vibrio anguillarum M3 strain genomic DNA through bacterial conjugation and homologous recombination. A mutant V anguillarum strain with angR gene mutation was constructed and screened successfully using TCBS medium containing chloramphenicol. PCR identification and sequencing showed that the recombinant plasmid inserted into Vibrio anguillarum genome DNA, as expected design, resulted in insertional inactivation of angR gene. Results of artificial infection experiment showed that virulence of mutant Vibrio anguillarum strain reduced dramatically as compared with the wild strain M3.
收录类别	SCI
语种	英语
WOS记录号	WOS:000358754200014
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/49673]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhendong,Mo, Zhaolan,Chen, Shiyong,et al. Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation[J]. JOURNAL OF ENVIRONMENTAL BIOLOGY,2015,36(2015):807-811.
APA	Zhang, Zhendong,Mo, Zhaolan,Chen, Shiyong,Zou, YuXia,&Zhang, Peijun.(2015).Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation.JOURNAL OF ENVIRONMENTAL BIOLOGY,36(2015),807-811.
MLA	Zhang, Zhendong,et al."Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation".JOURNAL OF ENVIRONMENTAL BIOLOGY 36.2015(2015):807-811.