Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted | |
Bao, Shaopan1,2; Lu, Qicong1,2; Fang, Tao1; Dai, Heping1; Zhang, Chao1,2 | |
刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
2015-12-01 | |
卷号 | 81期号:23页码:8098-8107 |
ISSN号 | 0099-2240 |
英文摘要 | To develop applicable and susceptible models to evaluate the toxicity of nanoparticles, the antimicrobial effects of CuO nanoparticles (CuO-NPs) on various Saccharomyces cerevisiae (S. cerevisiae) strains (wild type, single-gene-deleted mutants, and multiple-gene-deleted mutants) were determined and compared. Further experiments were also conducted to analyze the mechanisms associated with toxicity using copper salt, bulk CuO (bCuO), carbon-shelled copper nanoparticles (C/Cu-NPs), and carbon nanoparticles (C-NPs) for comparisons. The results indicated that the growth inhibition rates of CuO-NPs for the wild-type and the single-gene-deleted strains were comparable, while for the multiple-gene deletion mutant, significantly higher toxicity was observed (P < 0.05). When the toxicity of the CuO-NPs to yeast cells was compared with the toxicities of copper salt and bCuO, we concluded that the toxicity of CuO-NPs should be attributed to soluble copper rather than to the nanoparticles. The striking difference in adverse effects of C-NPs and C/Cu-NPs with equivalent surface areas also proved this. A toxicity assay revealed that the multiple-gene-deleted mutant was significantly more sensitive to CuO-NPs than the wild type. Specifically, compared with the wild-type strain, copper was readily taken up by mutant strains when cell permeability genes were knocked out, and the mutants with deletions of genes regulated under oxidative stress (OS) were likely producing more reactive oxygen species (ROS). Hence, as mechanism-based gene inactivation could increase the susceptibility of yeast, the multiple-gene-deleted mutants should be improved model organisms to investigate the toxicity of nanoparticles. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
关键词[WOS] | METAL-OXIDE NANOPARTICLES ; DISSOLVED ORGANIC-MATTER ; CELLS IN-VITRO ; OXIDATIVE STRESS ; CARBON NANOTUBES ; GENOTOXIC AGENTS ; PLASMA-MEMBRANE ; TESTING SYSTEM ; CWP GENES ; YEAST |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000364775700014 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/27359] |
专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Bao, Shaopan,Lu, Qicong,Fang, Tao,et al. Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(23):8098-8107. |
APA | Bao, Shaopan,Lu, Qicong,Fang, Tao,Dai, Heping,&Zhang, Chao.(2015).Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(23),8098-8107. |
MLA | Bao, Shaopan,et al."Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.23(2015):8098-8107. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论