Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS

	Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS
	Liang, Si-Cheng 1; Ge, Guang-Bo 1; Liu, Hui-Xin 1; Shang, Hai-Tao 2; Wei, Hong 2; Fang, Zhong-Ze 1; Zhu, Liang-Liang 1; Mao, Yu-Xi 1; Yang, Ling 1
刊名	journal of pharmaceutical and biomedical analysis
	2011-01-05
卷号	54 期号:1 页码:236-241
关键词	Ultra-fast liquid chromatography Propofol O-glucuronide Hepatic microsomes Interspecies difference Human individual difference
英文摘要	propofol o-glucuronidation has been used as probe reaction to phenotype ugt1a9 activity in human liver, thus a sensitive and specific method for determination of propofol o-glucuronide (pg) is urgently desirable. in the current study, a new lc-esi-ms method for determination of pg in hepatic microsomes from human (hlm), monkey (cylm), dog (dlm), minipig (plm), rat (rlm) and mouse (mlm) was developed and validated using 4-methylumbelliferyl-beta-d-glucuronide as an internal standard (is). pg and is was separated by a shim-pack xr-ods column (100 mm x 2.0 mm, 2.2 mu m, shimadzu) under gradient conditions with the mobile phase of acetonitrile and water containing 0.2% acetic acid (v/v). the mass spectrometric detection was performed under selected ion monitoring (sim) for pg at m/z 353 and is at m/z 351. the assay exhibited linearity over the range 0.05-30 mu m for pg with the correlation coefficient of 0.9995. the intra- and inter-day precision was less than 7.2%, with accuracy in the range 93.8-107.5%. the developed method was successfully used for characterizing interspecies and human individual differences in the o-glucuronidation activity towards propofol, as well as investigating inhibitory effects of androsterone and phenylbutazone on propofol o-glucuronidation in hlm. (c) 2010 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	liquid-chromatographic assay ; drug glucuronidation ; liver-microsomes ; metabolites ; urine ; pharmacokinetics ; prediction
收录类别	SCI
语种	英语
WOS记录号	WOS:000282863300037
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142726]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China 2.Third Mil Med Univ, Chongqing, Peoples R China
推荐引用方式 GB/T 7714	Liang, Si-Cheng,Ge, Guang-Bo,Liu, Hui-Xin,et al. Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS[J]. journal of pharmaceutical and biomedical analysis,2011,54(1):236-241.
APA	Liang, Si-Cheng.,Ge, Guang-Bo.,Liu, Hui-Xin.,Shang, Hai-Tao.,Wei, Hong.,...&Yang, Ling.(2011).Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS.journal of pharmaceutical and biomedical analysis,54(1),236-241.
MLA	Liang, Si-Cheng,et al."Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS".journal of pharmaceutical and biomedical analysis 54.1(2011):236-241.