Urinary metabonomics study in a rat model in response to protein-energy malnutrition by using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry

	Urinary metabonomics study in a rat model in response to protein-energy malnutrition by using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry
	Wu, Zeming 2; Li, Min 1; Zhao, Chunxia 2; Zhou, Jia 2; Chang, Yuwei 2; Li, Xiang 2; Gao, Peng 2; Lu, Xin 2; Li, Yousheng 1; Xu, Guowang 2
刊名	molecular biosystems
	2010
卷号	6 期号:11 页码:2157-2163
英文摘要	systematic studies were performed on the biological perturbations in metabolic phenotype responding to protein-energy malnutrition through global metabolic profiling analysis, in combination with pattern recognition. the malnutrition rat model was established through five weeks of strict diet restriction, and the metabonome data obtained from gas chromatography-mass spectrometry (gc-ms) and liquid chromatography-mass spectrometry (lc-ms) were integrated to approximate the comprehensive metabolic signature. principal component analysis and orthogonal projection to latent structure analysis were used for the classification of metabolic phenotypes and discovery of differentiating metabolites. the perturbations in the urine profiles of malnourished rats were marked by higher levels of creatine, threitol, pyroglutamic acid, gluconic acid and kynurenic acid, as well as decreased levels of succinic acid, cis-aconitic acid, citric acid, isocitric acid, threonic acid, trimethylglycine, n-methylnicotinic acid and uric acid. the alterations in these metabolites were associated with perturbations in energy metabolism, carbohydrate, amino acid, and fatty acid metabolism, purine metabolism, cofactor and vitamin metabolism, in response to protein and energy malnutrition. our findings show the integration of gc-ms and lc-ms techniques for untargeted metabolic profiling analysis was promising for nutriology.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	caloric restriction ; gc-ms ; hplc-ms ; lc-ms ; diet ; metabolomics ; discovery ; strategy ; dog
收录类别	SCI
语种	英语
WOS记录号	WOS:000282765000009
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141986]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nanjing Univ, Sch Med, Jinling Hosp, Dept Surg, Nanjing 210002, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wu, Zeming,Li, Min,Zhao, Chunxia,et al. Urinary metabonomics study in a rat model in response to protein-energy malnutrition by using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry[J]. molecular biosystems,2010,6(11):2157-2163.
APA	Wu, Zeming.,Li, Min.,Zhao, Chunxia.,Zhou, Jia.,Chang, Yuwei.,...&Xu, Guowang.(2010).Urinary metabonomics study in a rat model in response to protein-energy malnutrition by using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry.molecular biosystems,6(11),2157-2163.
MLA	Wu, Zeming,et al."Urinary metabonomics study in a rat model in response to protein-energy malnutrition by using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry".molecular biosystems 6.11(2010):2157-2163.