Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device

	Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device
	Ma, Huipeng 1,2; Liu, Tingjiao 1,3; Qin, Jianhua 1; Lin, Bingcheng 1
刊名	electrophoresis
	2010-05-01
卷号	31 期号:10 页码:1599-1605
关键词	Co-culture Microfluidic Migration Transdifferentiation
英文摘要	fibroblasts and tumor cells have been involved in the process of cancer development, progression and therapy. here, we present a simple microfluidic device which enables to study the interaction between fibroblasts and tumor cells by indirect contact co-culture. the device is composed of multiple cell culture chambers which are connected by a parallel of cell migration regions, and it enables to realize different types of cells to communicate each other on the single device. in this work, human embryonic lung fibroblasts cells were observed to exhibit obvious migration towards tumor cells instead of normal epithelial cells on the co-culture device. moreover, transdifferentiation of human embryonic lung fibroblast cells was recognized by the specific expression of a-smooth musle actin, indicating the effect of tumor cells on the behavior of fibroblasts. furthermore, multiple types of cell co-culture can be demonstrated on the single device which enables to mimic the complicated microenviroment in vivo. the device is simple and easy to operate, which enables to realize real-time observation of cell migration after external stimulus. this microfluidic device allows for the characterization of various cellular events on a single device sequentially, faciliating the better understanding of interaction between heterotypic cells in a more complex microenvironment.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	myofibroblast differentiation ; cancer ; microenvironment ; invasion ; stroma ; chip ; angiogenesis ; separation ; tissue
收录类别	SCI
语种	英语
WOS记录号	WOS:000278650500001
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141978]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Ma, Huipeng,Liu, Tingjiao,Qin, Jianhua,et al. Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device[J]. electrophoresis,2010,31(10):1599-1605.
APA	Ma, Huipeng,Liu, Tingjiao,Qin, Jianhua,&Lin, Bingcheng.(2010).Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device.electrophoresis,31(10),1599-1605.
MLA	Ma, Huipeng,et al."Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device".electrophoresis 31.10(2010):1599-1605.