A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis

	A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis
	Pan, Xiaoyan 1,2; Jiang, Lei 1; Liu, Kaiying 1; Lin, Bingcheng 1; Qin, Jianhua 1
刊名	analytica chimica acta
	2010-07-26
卷号	674 期号:1 页码:110-115
关键词	Microfluidic Parallel analysis Multichamber polymerase chain reaction Pathogen detection Genotyping
英文摘要	this work describes a microfluidic device integrated with multichamber polymerase chain reaction (pcr) and multichannel separation for parallel genetic analysis. the microdevice consists of three functional units: temperature control, multiple pcr (four chambers pcr), and multiple channel separation (four separation channels, each channel connected to a pcr chamber). platinum (pt)/titanium (ti) microheater was used to ensure homogeneous temperature field, and pt-chip sensor was used for temperature monitoring. the interface between chip-pcr and chip separation was simplified by connecting the pcr chamber with separation channel directly. after chip-pcr, pcr products were introduced into parallel separation channels for subsequent separation/detection by applying an electric field automatically. this microdevice was successfully applied for detection of pathogens including hepatitis b virus (hbv) and mycobacterium tuberculosis (mtb), and genotyping of human leucocyte antigen (hla)-b27 as well, demonstrating the feasibility of the integrated microdevice for parallel genetic analysis. (c) 2010 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	capillary electrophoresis microsystem ; analysis system ; pcr amplification ; dna amplification ; chip ; microdevice ; extraction ; microchip ; samples
收录类别	SCI
语种	英语
WOS记录号	WOS:000280570400016
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141790]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Pan, Xiaoyan,Jiang, Lei,Liu, Kaiying,et al. A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis[J]. analytica chimica acta,2010,674(1):110-115.
APA	Pan, Xiaoyan,Jiang, Lei,Liu, Kaiying,Lin, Bingcheng,&Qin, Jianhua.(2010).A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis.analytica chimica acta,674(1),110-115.
MLA	Pan, Xiaoyan,et al."A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis".analytica chimica acta 674.1(2010):110-115.