Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization

	Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization
	Lin, Zian 1,2; Yang, Fan 1; He, Xiwen 1; Zhang, Yukui 1,3
刊名	journal of separation science
	2009-11-01
卷号	32 期号:22 页码:3980-3987
关键词	Covalent immobilization Molecular imprinting Organic-inorganic hybrid Protein Sol-gel
英文摘要	the synthesis of poly-aminophenylboronic acid (apba) imprinted hybrid silica-based polymers for selective recognition of bovine hemoglobin (bhb) was described, where the mesoporous hybrid silica supporting matrices were prepared by a mild sol-gel process with tetraethoxysilane and 3-aminopropyltriethoxysilane as two precursors. covalent immobilization of bhb was adopted in order to create homogeneous recognition sites. after removal of the template, the resulting imprinted polymers showed high binding affinity toward bhb and the imprinting factor (alpha) reached 2.12. the specificity of the bhb recognition was evaluated with competitive experiments, indicating the imprinted polymers have a higher selectivity for the template bhb. the easy preparation protocol and good protein recognition proper-ties made the approach an attractive solution to depletion of high-abundance protein from bovine blood.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	molecularly imprinted polymers ; solid-phase extraction ; serum-albumin ; thin-films ; catalysis ; surfaces
收录类别	SCI
语种	英语
WOS记录号	WOS:000273993500016
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141341]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China 2.Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Lin, Zian,Yang, Fan,He, Xiwen,et al. Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization[J]. journal of separation science,2009,32(22):3980-3987.
APA	Lin, Zian,Yang, Fan,He, Xiwen,&Zhang, Yukui.(2009).Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization.journal of separation science,32(22),3980-3987.
MLA	Lin, Zian,et al."Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization".journal of separation science 32.22(2009):3980-3987.