Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography

	Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography
	Qu, Qishu ; Tang, Xiaoqing ; Mangelings, Debby ; Wang, Chengyin ; Yang, Gongjun ; Hu, Xiaoya ; Yan, Chao
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2007-06-15
卷号	853 期号:1-2 页码:31-37
关键词	micellar electrokinetic chromatography amino acids electroosmotic flow cation additive
英文摘要	the effect of a divalent cation (mg2+) and 3 monovalent cations (na+, li+, and k+) as buffer additives on the electroosmotic flow (eof) was investigated in order to improve the separation performance of p-acetamidobenzenesulfonyl fluoride (paabs-f) derivatives of 20 standard amino acids by micellar electrokinetic chromatography (mekc). the eof can be decreased with increasing concentration of cations with the order of cations as mg2+ > k+ > na+ > li+. however, it was found that the resolution cannot be improved easily using a buffer cation which is more capable of decreasing the eof. taking the migration time, resolution, and peak shape into account, na+ is the best buffer additive, although the eof decreased most using mg2+. using 20 mmol/l borate at ph 9.3 containing 140 mmol/l sds and 20 mmol/l na+ as electrolyte, 20 standard amino acids were successfully separated within 14 min. (c) 2007 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	capillary-zone-electrophoresis ; induced fluorescence detection ; precolumn derivatization ; liquid-chromatography ; aliphatic-amines ; buffer cation ; proteins ; reagent ; phenylisothiocyanate ; derivatives
收录类别	SCI
语种	英语
WOS记录号	WOS:000247704200004
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140472]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Yangzhou Univ, Coll Chem & Chem Engn, Jiangsu Key Lab Environm Mat & Environm Engn, Yangzhou 250002, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Ctr, Dalian 116023, Peoples R China 3.Free Univ Brussels, Dept Analyt Chem & Pharmaceut Technol, B-1090 Brussels, Belgium
推荐引用方式 GB/T 7714	Qu, Qishu,Tang, Xiaoqing,Mangelings, Debby,et al. Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2007,853(1-2):31-37.
APA	Qu, Qishu.,Tang, Xiaoqing.,Mangelings, Debby.,Wang, Chengyin.,Yang, Gongjun.,...&Yan, Chao.(2007).Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography.journal of chromatography b-analytical technologies in the biomedical and life sciences,853(1-2),31-37.
MLA	Qu, Qishu,et al."Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography".journal of chromatography b-analytical technologies in the biomedical and life sciences 853.1-2(2007):31-37.