The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations

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	The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations
	Hua, Jianghuan 1,2; Han, Jian 3; Guo, Yongyong 1; Zhou, Bingsheng 1
刊名	AQUATIC TOXICOLOGY
	2015-09-01
卷号	166 页码:1-9
关键词	Progestin Levonorgestrel Sex differentiation Sex ratio Androgenic activity Zebrafish
ISSN号	0166-445X
英文摘要	Synthetic progestins have become widespread environmental contaminants and may cause adverse effects on fish. In the present study, we investigated the effects of levonorgestrel (LNG) on sex differentiation in zebrafish (Danio rerio). Embryos were exposed to LNG at environmentally relevant concentrations (0, 1, 10, 33, and 100 ng/L) and allowed to develop until sexual maturity. Histological examination at 63 days post fertilization (dpf) caused complete sex reversal and 100% males were observed in the 10,33 and 100 ng/L treatments; gross morphological and histological examination of gonads at 142 dpf further confirmed 100% males at these exposure concentrations. The results indicate androgenic activity of LNG, and masculinization during zebrafish gonadal differentiation. The mRNA expression levels of genes involved in fish sex differentiation and gonadal development were examined at 28 and 42 dpf. Down-regulation of the mRNA expression of aromatase (e.g., cyp19a1 a, cyp19a1b), the forkhead transcription factor gene L2 (foxl2) and the Fushi tarazu factor-1d (nr5a1b) were observed. In contrast, transcription of the doublesex and mab-3-related transcription factor 1 (dmrt1) gene was up-regulated. Androgen receptor (ar) mRNA expression was significantly down-regulated at 28 and 42 dpf. Co-exposure to flutamide (an androgen antagonist) and LNG, led to a decrease in the sex inversion potency of LNG. Our study has demonstrated that environmentally relevant concentrations of LNG could alter sex differentiation and gonadal development in zebrafish. Our results also suggest a potentially high ecological risk of LNG to fish populations in LNG-contaminated aquatic environments. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology ; Toxicology
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
关键词[WOS]	MINNOW PIMEPHALES-PROMELAS ; STICKLEBACK GASTEROSTEUS-ACULEATUS ; ADVERSELY AFFECT REPRODUCTION ; AROMATASE GENE-TRANSCRIPTION ; DANIO-RERIO ; SYNTHETIC PROGESTINS ; OVARIAN-FOLLICLES ; BINARY-MIXTURES ; XENOPUS-LAEVIS ; FOXL2 GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000361256600001
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27210]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shangdong Acad Sci, Inst Biol, Jinan 250014, Peoples R China
推荐引用方式 GB/T 7714	Hua, Jianghuan,Han, Jian,Guo, Yongyong,et al. The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations[J]. AQUATIC TOXICOLOGY,2015,166:1-9.
APA	Hua, Jianghuan,Han, Jian,Guo, Yongyong,&Zhou, Bingsheng.(2015).The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations.AQUATIC TOXICOLOGY,166,1-9.
MLA	Hua, Jianghuan,et al."The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations".AQUATIC TOXICOLOGY 166(2015):1-9.