Preparation and time-resolved fluorometric application of luminescent europium nanoparticles

	Preparation and time-resolved fluorometric application of luminescent europium nanoparticles
	Tan, MQ; Ye, ZQ; Wang, GL; Yuan, JL
刊名	chemistry of materials
	2004-06-15
卷号	16 期号:12 页码:2494-2498
英文摘要	novel silica-coated luminescent europium (scle) nanoparticles have been prepared by copolymerization of tetraethyl orthosilicate and 3-aminopropyl(triethoxy)silane with ammonium hydroxide in a water-in-oil microemulsion containing a luminescent eu3+ chelate, triton x-100, n-octanol, and cyclohexane. the nanoparticles were characterized by transmission electron microscopy, x-ray diffraction, and uv-vis and luminescence spectra. the results show that the nanoparticles are spherical and uniform in size, 50 +/- 5 nm in diameter, and have high photostability and long luminescence lifetime (770 mus). because primary amino groups have been directly introduced to the nanoparticle's surface by using 3-aminopropyl-(triethoxyl)silane in nanoparticle preparation, the surface modification and bioconjugation of the nanoparticles are easier and reproducible. the nanoparticles were used for streptavidin (sa) labeling, and the nanoparticle-labeled streptavidin was successfully used in time-resolved fluoroimmunoassay of human a-fetoprotein. the results reveal that the new nanoparticles are favorable for use as a new type of luminescence probe for highly sensitive time-resolved luminescence bioassay.
WOS标题词	science & technology ; physical sciences ; technology
类目[WOS]	chemistry, physical ; materials science, multidisciplinary
研究领域[WOS]	chemistry ; materials science
关键词[WOS]	doped silica nanoparticles ; prostate-specific antigen ; dna hybridization ; ultrasensitive detection ; bioanalytical assays ; fluorescent chelate ; fluoroimmunoassay ; immunoassays ; probes ; biomolecules
收录类别	SCI
语种	英语
WOS记录号	WOS:000221976400023
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139350]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
推荐引用方式 GB/T 7714	Tan, MQ,Ye, ZQ,Wang, GL,et al. Preparation and time-resolved fluorometric application of luminescent europium nanoparticles[J]. chemistry of materials,2004,16(12):2494-2498.
APA	Tan, MQ,Ye, ZQ,Wang, GL,&Yuan, JL.(2004).Preparation and time-resolved fluorometric application of luminescent europium nanoparticles.chemistry of materials,16(12),2494-2498.
MLA	Tan, MQ,et al."Preparation and time-resolved fluorometric application of luminescent europium nanoparticles".chemistry of materials 16.12(2004):2494-2498.