Analysis of bile acids by high performance liquid chromatography after pre-column derivatization with 9-(2-hydroxyethyl)-acridone

	Analysis of bile acids by high performance liquid chromatography after pre-column derivatization with 9-(2-hydroxyethyl)-acridone
	Zhang, L ; You, JM ; Zhang, X ; Tian, Y ; Duan, JC ; Liang, Z ; Zhang, WB ; Yan, C ; Zhang, YK
刊名	chinese journal of analytical chemistry
	2004-09-01
卷号	32 期号:9 页码:1131-1134
关键词	bile acids derivatization fluorescence detection high performance liquid chromatography
英文摘要	9-(2-hydroethyl) -acridone (hea), a novel ultraviolet and fluorescence labelling reagent, has been utilized as pre-column derivatization reagent for the analysis of 10 bile acids by high performance liquid chromatography ( hplc) with fluorescence detection. hea reacts with bile acids in the presence of the coupling agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride ( edc . hcl) and the base catalyst 4-dimethylaminopyridine ( dmap) to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 404 nm and an emission maximum at lambda(em) 440 nm. the labeled derivatives exhibit high stability under reversed-phase conditions. the method, in conjunction with a gradient elution, offers a baseline resolution of the 10 bile acids derivatives on a reversed-phase hypersil bds c-18 column. the average detection limit ( at a signal-to-noise ratio of 3) for per injection is 8.3 fmol.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000224542700002
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139334]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Qufu Normal Univ, Dept Chem, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	Zhang, L,You, JM,Zhang, X,et al. Analysis of bile acids by high performance liquid chromatography after pre-column derivatization with 9-(2-hydroxyethyl)-acridone[J]. chinese journal of analytical chemistry,2004,32(9):1131-1134.
APA	Zhang, L.,You, JM.,Zhang, X.,Tian, Y.,Duan, JC.,...&Zhang, YK.(2004).Analysis of bile acids by high performance liquid chromatography after pre-column derivatization with 9-(2-hydroxyethyl)-acridone.chinese journal of analytical chemistry,32(9),1131-1134.
MLA	Zhang, L,et al."Analysis of bile acids by high performance liquid chromatography after pre-column derivatization with 9-(2-hydroxyethyl)-acridone".chinese journal of analytical chemistry 32.9(2004):1131-1134.