Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers

	Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers
	Yang, L; Jia, LY; Zou, HF; Zhou, DM; Zhang, YK
刊名	science in china series b-chemistry
	1998-12-01
卷号	41 期号:6 页码:596-605
关键词	membrane affinity chromatography matrix separation biopolymers
英文摘要	a new iminodiacetic acid (ida) type chelating membrane based on composite matrix formed by covalently coupling the polyglycidyl methacrylate to cotton cellulose fiber was developed. the study focused on the optimal conditions for the preparation of the membrane and the reaction of the chelating agent (iminodiacetic acid, ida) with the matrix. the physical properties of the chelating membrane were tested by electron micrography and bet surface area instrument. the relationship between flow-rate and backpressure was almost linear. adsorption isotherm of protein on cu2+ - ida membrane was determined by batch experiments. the dissociation constants and the maximum adsorption capacity were obtained. the results of affinity purification of human serum albumin and bovine liver catalase on membrane are comparable with those on gel chromatography presented in literature. the separations performed on the membrane chromatography, however, were 3-5 times faster than those on gel chromatography. the immobilized metal composite membrane was also successfully used in hplc for rapid analysis of protein. thus, the composite membrane reported here, with low backpressure, low cost and long service-life, is quite suitable for rapid and large scale affinity purification and hplc analysis of biopolymers.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	chromatography ; purification ; proteins
收录类别	SCI
语种	英语
WOS记录号	WOS:000077599300005
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138470]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China
推荐引用方式 GB/T 7714	Yang, L,Jia, LY,Zou, HF,et al. Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers[J]. science in china series b-chemistry,1998,41(6):596-605.
APA	Yang, L,Jia, LY,Zou, HF,Zhou, DM,&Zhang, YK.(1998).Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers.science in china series b-chemistry,41(6),596-605.
MLA	Yang, L,et al."Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers".science in china series b-chemistry 41.6(1998):596-605.