Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions

	Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions
	Han, FT; Xue, J; Lin, BC
刊名	talanta
	1998-08-01
卷号	46 期号:4 页码:735-742
关键词	capillary electrophoresis DNA separation mannitol
英文摘要	a new kind of sieving matrix is presented in this paper to allow satisfactory separation of dna fragments in a relatively low viscous solution. when a certain amount of mannitol was added to cellulose solution not concentrated enough to separate pgem-3zf(+)/haeiii standards well, a polymer solution with low viscosity but with very good separation effects was obtained. the separation result of this sieving buffer was comparable with those using highly concentrated cellulose solutions. the sieving ability of solutions with different cellulose concentrations and different amounts of mannitol has been investigated. it was proved that 0.5% was the minimum hydroxypropylmethylcellulose (hpmc) concentration that could be used to separate dna fragments satisfactorily. hpmc solutions with a concentration of less than 0.5% could not separate the standard dna fragments even in the presence of mannitol. it was found that 6% was the optimized mannitol concentration because either more or less mannitol will lead a decrease of resolution. the principle of the positive influence of mannitol has also been discussed. (c) 1998 elsevier science b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	polymorphism analysis ; chain-reaction ; restriction fragments ; products
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000074745200033
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138193]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Han, FT,Xue, J,Lin, BC. Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions[J]. talanta,1998,46(4):735-742.
APA	Han, FT,Xue, J,&Lin, BC.(1998).Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions.talanta,46(4),735-742.
MLA	Han, FT,et al."Mannitol influence on the separation of DNA fragments by capillary electrophoresis in entangled polymer solutions".talanta 46.4(1998):735-742.