Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography

	Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography
	Ding Ling; Dong Jun; Xiao Yuan-Sheng; Zhang Xiu-Li; Xue Xing-Ya; Liang Xin-Miao
刊名	chinese journal of analytical chemistry
	2013-02-01
卷号	41 期号:2 页码:181-186
关键词	Protein Reversed phase liquid chromatography Retention equation Predict Optimization
英文摘要	study on the retention equation for protein in rplc can contribute to optimizing gradient conditions for protein separation. in this study, five standard proteins, insulin, cytochrome c, lysozyme, transferrin and myoglobin were selected, and their retention time on three reversed-phase columns (waters symmetry 300 c-4, waters symmetry 300 c-18, c8hc) under six different linear gradients was obtained. according to nonlinear curve fit (csass software), the retention equation for five standard proteins on three columns was established. the regression coefficients of the 15 equations were all above 0: 999, indicating that the equation could accurately describe protein retention behavior on rplc mode. based on the equation, retention time for 5 standard proteins under other gradient conditions was predicted, and the relative errors for each protein were below 5%. this retention equation could be used in optimizing gradient conditions for protein separation on rplc, leading to rapid baseline separation of five mixed standard proteins within 20 min.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	hydrophobic interaction chromatography ; affinity-chromatography ; purification ; separation ; gradient ; systems ; elution
收录类别	SCI
语种	英语
WOS记录号	WOS:000318392300004
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137791]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ding Ling,Dong Jun,Xiao Yuan-Sheng,et al. Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography[J]. chinese journal of analytical chemistry,2013,41(2):181-186.
APA	Ding Ling,Dong Jun,Xiao Yuan-Sheng,Zhang Xiu-Li,Xue Xing-Ya,&Liang Xin-Miao.(2013).Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography.chinese journal of analytical chemistry,41(2),181-186.
MLA	Ding Ling,et al."Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography".chinese journal of analytical chemistry 41.2(2013):181-186.