Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification

	Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification
	Long, Zhen; Guo, Zhimou; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
刊名	journal of separation science
	2013-12-01
卷号	36 期号:24 页码:3845-3852
关键词	Alkaloids Orthogonality Strong cation exchange Symmetrical peaks
英文摘要	peak tailing and nonalkaloid coelution usually hinder alkaloid purification. in this study, a 2dlc, strong cation exchange (scx) coupled with positively charged rp (pgrp) lc, was developed to overcome these problems. ten compounds including basic and nonbasic compounds were analyzed. nonbasic compounds, which are coeluted with basic compounds on rp or pgrp columns, were weakly retained on the scx column. in addition, a symmetrical peak shape (tailing factors <1.2) of basic compounds can be obtained in the current system. compared to two other 2d systems, the current system provided the highest orthogonality (r-2 = 0.045). furthermore, the scx coupled with pgrp system was applied for alkaloid purification from a traditional chinese medicine. nineteen alkaloids were obtained and one of them was identified as a novel compound. the overall results demonstrate that the proposed system is a powerful tool for alkaloid purification.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	w. t. wang ; mass-spectrometric detection ; basic compounds ; stationary-phase ; capillary-electrophoresis ; sample preparation ; separation ; performance ; hplc ; extraction
收录类别	SCI
语种	英语
WOS记录号	WOS:000328138200005
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137665]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification[J]. journal of separation science,2013,36(24):3845-3852.
APA	Long, Zhen,Guo, Zhimou,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2013).Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification.journal of separation science,36(24),3845-3852.
MLA	Long, Zhen,et al."Two-dimensional strong cation exchange/positively charged reversed-phase liquid chromatography for alkaloid analysis and purification".journal of separation science 36.24(2013):3845-3852.