Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA

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	Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA
	Guan, Lingmei 1; Huang, Saipeng 2; Chen, Zhao 3; Li, Yanchao 2; Liu, Ke 1; Liu, Yang 2; Du, Libo 2
刊名	JOURNAL OF NANOPARTICLE RESEARCH
	2015-09-25
卷号	17 期号:9
关键词	Gene carriers Nanoparticles PAMAM siRNA delivery Fluorescence Drug delivery
英文摘要	Visual detection of gene vectors has attracted a great deal of attention due to the application of these vectors in monitoring and evaluating the effect of gene carriers in living cells. A non-viral vector, the fluorescent PAMAM dendrimer (F-PAMAM), was synthesized through conjugation of PAMAM dendrimers and fluorescein. In vitro and ex vivo experiments show that F-PAMAM exhibits superphotostability, low cytotoxicity and facilitates endocytosis by A549 cells. The vector has a high siRNA binding affinity and it increases the efficiency of cy5-siRNA delivery in A549 cells, in comparison with a cy5-siRNA monomer. Our results provide a new method for simultaneously monitoring the delivery of siRNA and its non-viral carriers in living cells.
收录类别	SCI
语种	英语
公开日期	2015-11-03
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28428]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Sichuan Univ, State Key Lab Bioresources & Ecoenvironm, Minist Educ, Coll Life Sci, Chengdu 610064, Peoples R China 2.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Ctr Mol Sci, Beijing 100190, Peoples R China 3.Xi An Jiao Tong Univ, Sch Sci, Xian 710049, Peoples R China
推荐引用方式 GB/T 7714	Guan, Lingmei,Huang, Saipeng,Chen, Zhao,et al. Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA[J]. JOURNAL OF NANOPARTICLE RESEARCH,2015,17(9).
APA	Guan, Lingmei.,Huang, Saipeng.,Chen, Zhao.,Li, Yanchao.,Liu, Ke.,...&Du, Libo.(2015).Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA.JOURNAL OF NANOPARTICLE RESEARCH,17(9).
MLA	Guan, Lingmei,et al."Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA".JOURNAL OF NANOPARTICLE RESEARCH 17.9(2015).