New photostable naphthalimide-based fluorescent probe for mitochondrial imaging and tracking

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	New photostable naphthalimide-based fluorescent probe for mitochondrial imaging and tracking
	Huang, Saipeng 1,2; Han, Rongcheng 3; Zhuang, Qianfen 1; Du, Libo 1; Jia, Hongying 1; Liu, Yangping 4; Liu, Yang 1
刊名	BIOSENSORS & BIOELECTRONICS
	2015-09-15
卷号	71 页码:313-321
关键词	Fluorescent probe Mitochondrial imaging 1 Photostability Real-time tracking 8-naphthalimides
英文摘要	Monitoring mitochondria morphological changes temporally and spatially exhibits significant importance for diagnosing, preventing and treating various diseases related to mitochondrial dysfunction. However, the application of commercially available mitochondria trackers is limited due to their poor photostability. To overcome these disadvantages, we designed and synthesized a mitochondria-localized fluorescent probe by conjugating 1,8-naphthalimide with triphenylphosphonium (i.e. NPA-TPP). The structure and characteristic of NPA-TPP was characterized by UV-vis, fluorescence spectroscopy, (HNMR)-H-1, (CNMR)-C-13, FTIR, MS, etc. The photostability and cell imaging were performed on the laser scanning confocal microscopy. Moreover, the cytotoxicity of NPA-TPP on cells was evaluated using (3-[4,5-dimethylthiazol-2-y1]-2,5 diphenyl tetrazolium bromide) assay. The results showed that NPA-TPP not only has high sensitivity and specificity to mitochondria, but also exhibits super-high photostability, negligible cytotoxicity and good water solubility. In short, NPA-TPP indicates great potential for targeting mitochondria and enables a real-time and long-term tracking mitochondrial dynamics changes. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2015-11-02
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/27947]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Res Resources Ctr, Beijing 100101, Peoples R China 4.Tianjin Med Univ, Sch Pharm, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Huang, Saipeng,Han, Rongcheng,Zhuang, Qianfen,et al. New photostable naphthalimide-based fluorescent probe for mitochondrial imaging and tracking[J]. BIOSENSORS & BIOELECTRONICS,2015,71:313-321.
APA	Huang, Saipeng.,Han, Rongcheng.,Zhuang, Qianfen.,Du, Libo.,Jia, Hongying.,...&Liu, Yang.(2015).New photostable naphthalimide-based fluorescent probe for mitochondrial imaging and tracking.BIOSENSORS & BIOELECTRONICS,71,313-321.
MLA	Huang, Saipeng,et al."New photostable naphthalimide-based fluorescent probe for mitochondrial imaging and tracking".BIOSENSORS & BIOELECTRONICS 71(2015):313-321.