Novel electrospray ionization-tandem mass spectrometry strategy for monitoring mercury(II) ion based on the competing system of mercury specific DNA and glutathione to mercury(II) ion

	Novel electrospray ionization-tandem mass spectrometry strategy for monitoring mercury(II) ion based on the competing system of mercury specific DNA and glutathione to mercury(II) ion
	Zhang, Ruixing ; Zhuang, Xiaoyu ; Liu, Shu ; Song,Rengrui ; Liu,Zhiqiang
刊名	analytical methods
	2014
卷号	6 期号:15 页码:5746-5752
关键词	FUNCTIONALIZED GOLD NANOPARTICLES AQUEOUS-SOLUTIONS SELECTIVE SENSOR HG-II FLUORESCENCE DESIGN CELLS APTASENSOR BIOSENSOR ASSAY
通讯作者	song,fr
英文摘要	an electrospray ionization-tandem mass spectrometry (esi-ms/ms) strategy was developed for the detection of mercury ion with high sensitivity and selectivity based on a competition system of glutathione and mercury specific dna to mercury ion. mercury ion selectively bound to a special mismatched dna and glutathione (gsh) because thiol-containing tripeptide can effectively sequester hg2+ ion from thymine-hg2+-thymine structure. following the competition reaction, the concentration of mercury ion in the analyte was indirectly reflected by monitoring the concentration change of free gsh by esi-ms/ms method. the electrospray ionization-mass spectrometry (esi-ms) analysis of the competition system provided the connection of mercury ion and mercury specific dna (msd), and also provided the process by which gsh competed mercury ion from msd. note that the proposed competing platform exhibits exquisite selectivity and sensitivity to hg2+ ion with a detection limit of 5 nm. furthermore, this assay design avoids the labeling of the probe, use of the quantum dot, fluorescence dye, and heavy metals. therefore, it is much friendlier for the operators and the environment. the proposed method was applied to some real samples (tap water, lake water, and fish), and the obtained results were satisfactory.
收录类别	SCI
语种	英语
WOS记录号	WOS:000339468600043
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57477]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhang, Ruixing,Zhuang, Xiaoyu,Liu, Shu,et al. Novel electrospray ionization-tandem mass spectrometry strategy for monitoring mercury(II) ion based on the competing system of mercury specific DNA and glutathione to mercury(II) ion[J]. analytical methods,2014,6(15):5746-5752.
APA	Zhang, Ruixing,Zhuang, Xiaoyu,Liu, Shu,Song,Rengrui,&Liu,Zhiqiang.(2014).Novel electrospray ionization-tandem mass spectrometry strategy for monitoring mercury(II) ion based on the competing system of mercury specific DNA and glutathione to mercury(II) ion.analytical methods,6(15),5746-5752.
MLA	Zhang, Ruixing,et al."Novel electrospray ionization-tandem mass spectrometry strategy for monitoring mercury(II) ion based on the competing system of mercury specific DNA and glutathione to mercury(II) ion".analytical methods 6.15(2014):5746-5752.