The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry

	The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry
	Yang, Dong ; Zhao, Jiadi ; Liu, Shu ; Song,Fengrui ; Liu,Zhiqiang
刊名	analytical methods
	2014
卷号	6 期号:10 页码:3353-3359
关键词	TYPE-2 DIABETES-MELLITUS METABOLOMICS IONIZATION
通讯作者	liu,s
英文摘要	alpha-glucosidase inhibitors have been used for the management of type 2 diabetes (t2dm) for a long time. the natural alpha-glucosidase inhibitors from chinese herbal medicine have become an attractive therapeutic approach for treating t2dm due to their low toxicity. in this study, a new kind of in vitro alpha-glucosidase inhibition assay based on using maltose as the substrate was developed, and this new established method was used to determine the alpha-glucosidase inhibitory activities of polygonum multiflorum and four anthraquinone compounds. as a result, polygonum multiflorum showed 50% alpha-glucosidase inhibition at the concentration of 0.0032 mg ml(-1) and four anthraquinone compounds including emodin, aloe-emodin, physcion and rhein showed strong alpha-glucosidase inhibitory activities with ic50 values ranging from 4.12 mu m to 5.68 mu m, respectively. moreover, the centrifugal ultrafiltration with lc-esi-msn was used to screen and identify active alpha-glucosidase inhibitors from polygonum multiflorum extract and nine small-molecule active compounds were successfully identified as potential alpha-glucosidase inhibitors. five compounds belonged to the anthraquinone group including emodin, physcion, aloe-emodin, rhein and physcion-8-o-glucoside, while the others were catechin, resveratrol, 2,3,5,4 '-tetrahydroxystilbene-2-o-glucoside and torachryson-8-o-glucoside.
收录类别	SCI
语种	英语
WOS记录号	WOS:000335224900025
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57475]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Yang, Dong,Zhao, Jiadi,Liu, Shu,et al. The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry[J]. analytical methods,2014,6(10):3353-3359.
APA	Yang, Dong,Zhao, Jiadi,Liu, Shu,Song,Fengrui,&Liu,Zhiqiang.(2014).The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry.analytical methods,6(10),3353-3359.
MLA	Yang, Dong,et al."The screening of potential alpha-glucosidase inhibitors from the Polygonum multiflorum extract using ultrafiltration combined with liquid chromatography-tandem mass spectrometry".analytical methods 6.10(2014):3353-3359.