A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols

	A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols
	Wu, Zhenzhu ; Li, Wenying ; Chen, Jian ; Yu,Cong
刊名	talanta
	2014
卷号	119 期号:4 页码:538-543
关键词	LABEL-FREE COLORIMETRIC PROBES EMERGENT NANOLIGHTS GOLD NANOPARTICLES MASS-SPECTROMETRY AQUEOUS-SOLUTIONS CARBON NANODOTS GLUTATHIONE CYSTEINE HOMOCYSTEINE
通讯作者	yu,c
英文摘要	a facile assay method for the highly sensitive and selective sensing of biothiols based on graphene quantum dots (gqds) has been developed. gqds emitted strong blue fluorescence in an aqueous buffer solution. it was observed that mercury(ii) ions could efficiently bind and quench the fluorescence of the gqds. when a biothiol compound (glutathione, cysteine, or homocysteine) was added to the assay mixture of gqds and mercury(ii), it bound to mercury(ii) ions. hg2+-gqd complex dissociated, and a fluorescence turn-on signal was detected. the emission intensity changes of the gqds could be directly related to the amount of biothiol added to the assay solution. the assay is highly sensitive, the limits of detection clod) for gsh, cys and hcy were 5 nm, 2.5 nm and 5 nm, respectively. the assay is also highly selective, a number of amino acids and proteins were tested, and little interference was observed. in addition, gsh standard recovery in serum samples was also demonstrated. we envision that our assay method could facilitate the biothiol quantification related biological and biomedical research. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000331670700077
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57324]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wu, Zhenzhu,Li, Wenying,Chen, Jian,et al. A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols[J]. talanta,2014,119(4):538-543.
APA	Wu, Zhenzhu,Li, Wenying,Chen, Jian,&Yu,Cong.(2014).A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols.talanta,119(4),538-543.
MLA	Wu, Zhenzhu,et al."A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols".talanta 119.4(2014):538-543.