Chemical profiling of Wu-tou decoction by UPLC-Q-TOF-MS

	Chemical profiling of Wu-tou decoction by UPLC-Q-TOF-MS
	Qi, Yao ; Li, Shizhe ; Pi, Zifeng ; Song,Fengrui ; Lin,Na ; Liu,Shu ; Liu,Zhaqiang
刊名	talanta
	2014
卷号	118 期号:1 页码:21-29
关键词	TANDEM-MASS-SPECTROMETRY PERFORMANCE LIQUID-CHROMATOGRAPHY TRADITIONAL CHINESE MEDICINE DIODE-ARRAY DETECTION ALKALOIDS CONSTITUENTS AGLYCONES FLAVONOL RADIX
通讯作者	liu,s
英文摘要	wu-tou decoction (wtd), a traditional chinese medicine (tcm) formula, is composed of aconiti radix cocta, ephedrae herba, paeoniae radix alba, astragali radix and glycyrrhiza radix preparata, and it has been used for more than a thousand years to treat rheumatic arthritis, rheumatoid arthritis and pain of joints, while the active constitutions of wtd are unclear. in this research, an ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (uplc-q-tof-ms) method in both positive and negative ion mode was established to investigate the major constitutions in wtd. a waters acquity uplc beh c18 column was used to separate the aqueous extract of wtd. acetonitrile and 0.1% aqueous formic acid (v/v) were used as the mobile phase. 74 components including alkaloids, monoterpene glycosides, triterpene saponins, flavones and flavone glycosides were identified or tentatively characterized in wtd based on the accurate mass within 15 ppm error and tandem ms behavior. all the constitutions were also detected in the corresponding individual herbs. these results will provide a basis for further study in vivo of wtd and the information of potential new drug structure for treating rheumatic arthritis and rheumatoid arthritis. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000329553600004
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57322]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Qi, Yao,Li, Shizhe,Pi, Zifeng,et al. Chemical profiling of Wu-tou decoction by UPLC-Q-TOF-MS[J]. talanta,2014,118(1):21-29.
APA	Qi, Yao.,Li, Shizhe.,Pi, Zifeng.,Song,Fengrui.,Lin,Na.,...&Liu,Zhaqiang.(2014).Chemical profiling of Wu-tou decoction by UPLC-Q-TOF-MS.talanta,118(1),21-29.
MLA	Qi, Yao,et al."Chemical profiling of Wu-tou decoction by UPLC-Q-TOF-MS".talanta 118.1(2014):21-29.