The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain

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	The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain
	Song, Y.1,2; Duan, X.1,2; Chen, J.1; Huang, W.1; Zhu, Z.1; Hu, W.1
刊名	JOURNAL OF NEUROENDOCRINOLOGY
	2015-03-01
卷号	27 期号:3 页码:198-211
关键词	Kiss1 Kiss2 GnRH3 transgenic zebrafish
ISSN号	0953-8194
通讯作者	Hu, W (reprint author), 7 Donghu South Rd, Wuhan 430072, Peoples R China.
英文摘要	Kisspeptin is a neuroendocrine hormone with a critical role in the activation of gonadotrophin-releasing hormone (GnRH) neurones, which is vital for the onset of puberty in mammals. However, the functions of kisspeptin neurones in non-mammalian vertebrates are not well understood. We have used transgenics to labell kisspeptin neurones (Kiss1 and Kiss2) with mCherry in zebrafish (Danio rerio). In kiss1:mCherry transgenic zebrafish, Kiss1 cells were located in the dorsomedial and ventromedial habenula, with their nerve fibres contributing to the fasciculus retroflexus and projecting to the ventral parts of the interpeduncular and raphe nuclei. In kiss2:mCherry zebrafish, Kiss2 cells were primarily located in the dorsal zone of the periventricular hypothalamus and, to a lesser extent, in the periventricular nucleus of the posterior tuberculum and the preoptic area. Kiss2 fibres formed a wide network projecting into the telencephalon, the mesencephalon, the hypothalamus and the pituitary. To study the relationship of kisspeptin neurones and GnRH3 neurones, these fish were crossed with gnrh3:EGFP zebrafish to obtain kiss1:mCherry/gnrh3:EGFP and kiss2:mCherry/gnrh3:EGFP double transgenic zebrafish. The GnRH3 fibres ascending to the habenula were closely associated with Kiss1 fibres projecting from the ventral habenula. On the other hand, GnRH3 fibres and Kiss2 fibres were adjacent but scarcely in contact with each other in the telencephalon and the hypothalamus. The Kiss2 and GnRH3 fibres in the ventral hypothalamus projected into the pituitary via the pituitary stalk. In the pituitary, Kiss2 fibres were directly in contact with GnRH3 fibres in the pars distalis. These results reveal the pattern of kisspeptin neurones and their connections with GnRH3 neurones in the brain, suggesting distinct mechanisms for Kiss1 and Kiss2 in regulating reproductive events in zebrafish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Endocrinology & Metabolism ; Neurosciences
研究领域[WOS]	Endocrinology & Metabolism ; Neurosciences & Neurology
关键词[WOS]	METASTASIS-SUPPRESSOR GENE ; PROTEIN-COUPLED RECEPTOR ; MEDAKA ORYZIAS-LATIPES ; GNRH NEURONS ; REPRODUCTIVE-BEHAVIOR ; KISS2 NEURONS ; MIDBRAIN TARGET ; MESSENGER-RNA ; SYSTEM ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000350146800003
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23907]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Song, Y.,Duan, X.,Chen, J.,et al. The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain[J]. JOURNAL OF NEUROENDOCRINOLOGY,2015,27(3):198-211.
APA	Song, Y.,Duan, X.,Chen, J.,Huang, W.,Zhu, Z.,&Hu, W..(2015).The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain.JOURNAL OF NEUROENDOCRINOLOGY,27(3),198-211.
MLA	Song, Y.,et al."The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain".JOURNAL OF NEUROENDOCRINOLOGY 27.3(2015):198-211.