Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)
	Feng, Xiu 1,2; Yu, Xiaomu 1; Pang, Meixia 1,2; Liu, Haiyang 1,2; Tong, Jingou 1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2015-04-01
卷号	182 页码:37-46
关键词	Cyprinus carpio Preprosomatostatin Molecular characterization Expression pattern Genetic association
ISSN号	1096-4959
通讯作者	Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
英文摘要	Somatostatins (SSs) are a structurally diverse family of peptides that play important roles in the regulation of growth, development and metabolism in vertebrates. In this study, three preprosomatostatin genes (PSSs) in the common carp, Cyprinus carpio (Cc) were identified and characterized. Based on cloned sequences and genome BLAST, six isoforms of the PSS gene in C. carpio (CcPSS) were identified and included CcPSS1a and CcPSS1b, CcPSS2a and CcPSS2b, and finally, CcPSS3a and CcPSS3b. The open reading frames (ORF) of CcPSS1a, CcPSS2a and CcPSS3a consist of 345, 336 and 363 nucleotides. During embryonic development, the expressions of CcPSS2 and CcPSS3 were first observed at the stage of optic vesicle, and CcPSS1 mRNA was initially detected at the stage of muscular effect. The highest mRNA levels of CcPSS1, CcPSS2 and CcPSS3 were observed at 1-day post-hatch (dph), 2-dph and the stage of heart beating, respectively. In the adult brain, the distributions of three CcPSS mRNAs were differential but overlapping in the hypothalamus, telencephalon and medulla oblongata. For peripheral tissues, all three CcPSS mRNAs were detected in the mid-intestine, and CcPSS1 and CcPSS3 mRNAs were also expressed in the liver. Owing to the importance of somatostatins on regulating growth, functional mutations of CcPSSs were identified in a C carpio population. A total of 23 polymorphic sites were detected in CcPSS1a and CcPSS3a. Of them, two SNPs (CcPSS1a-g.922C>T, and CcPSS3a-g.1125C>A) were significantly associated with growth traits, indicating their potential applications in gene (marker)-assisted selective breeding in C. carpio. (C) 2014 Elsevier Inc All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Zoology
关键词[WOS]	QUANTITATIVE TRAIT LOCI ; MESSENGER-RNAS ; SOMATOSTATIN PRECURSORS ; EMBRYONIC-DEVELOPMENT ; POLYGENIC EXPRESSION ; CARCASS COMPOSITION ; PROSOMATOSTATIN-I ; HORMONE RECEPTOR ; FISH ; CORTISTATIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000352746300005
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23897]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Feng, Xiu,Yu, Xiaomu,Pang, Meixia,et al. Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2015,182:37-46.
APA	Feng, Xiu,Yu, Xiaomu,Pang, Meixia,Liu, Haiyang,&Tong, Jingou.(2015).Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,182,37-46.
MLA	Feng, Xiu,et al."Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 182(2015):37-46.