Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection

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	Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection
	Jiang, Yao 1,3; He, Libo 1; Ju, Changsong 1,2; Pei, Yongyan 1,3; Ji, Myonghuan 2; Li, Yongming 1; Liao, Lanjie 1; Jang, Songhun 1,2; Zhu, Zuoyan 1; Wang, Yaping 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-05-01
卷号	44 期号:1 页码:88-99
关键词	Grass carp Toll-like receptor 5 Flagellin GCRV
ISSN号	1050-4648
通讯作者	Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
英文摘要	Toll-like receptor 5 (TLR5), a member of Toll-like receptors (TLRs) family and is responsible for the bacterial flagellin recognition in vertebrates, play an important role in innate immunity. In the study, two TLR5 genes of grass carp (Ctenopharyngodon idellus), named CiTLR5a and CiTLR5b, were cloned and analyzed. Both CiTLR5a and CiTLR5b are typical TLR proteins, including LRR motif, transmembrane region and TIR domain. The full-length cDNA of CiTLR5a is 3054 bp long, with a 2646 bp open reading frame (ORF), 78 bp 5' untranslated regions (UTR), and 330 bp 3' UTR. The full-length cDNA of CiTLR5b is 3326 bp, with a 2627 bp ORF, 95 bp 5' UTR, and 594 bp 3' UTR. Phylogenetic analysis showed that CiTLR5a and CiTLR5b were closed to the TLR5 of cirrhinus mrigala, cyprinus_carpio, and danio redo. Subcellular localization indicated that CiTLR5a and CiTLR5b shared similar localization pattern and may locate in the plasma membrane of transfected cells. Real-time quantitative PCR revealed CiTLR5a and CiTLR5b were constitutively expressed in all examined tissues, whereas the highest expressed tissue differed. Following exposure to flagellin and GCRV, CiTLR5a and CiTLR5b were up-regulated significantly. Moreover, the downstream genes of TLR5 signal pathway such as MyD88, NF-kappa B, IRF7, IL-1 beta, and TNF-alpha also up-regulated significantly, whereas the I kappa B gene was down-regulated, suggesting that CiTLR5a and CiTLR5b involved in response to flagellin stimulation and GCRV infection. The results obtained in the study would provide a new insight for further understand the function of TLR5 in teleost fish. (C) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	BACTERIAL FLAGELLIN ; SIGNALING PATHWAYS ; MOLECULAR-CLONING ; INNATE IMMUNITY ; BONY FISH ; IDENTIFICATION ; TLR5 ; RNA ; CANDIDATE ; PROFILES
收录类别	SCI
语种	英语
WOS记录号	WOS:000353743900011
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23894]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Kim Il Song Univ, Coll Life Sci, Dept Zool, Pyongyang, South Korea 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yao,He, Libo,Ju, Changsong,et al. Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(1):88-99.
APA	Jiang, Yao.,He, Libo.,Ju, Changsong.,Pei, Yongyan.,Ji, Myonghuan.,...&Wang, Yaping.(2015).Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection.FISH & SHELLFISH IMMUNOLOGY,44(1),88-99.
MLA	Jiang, Yao,et al."Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection".FISH & SHELLFISH IMMUNOLOGY 44.1(2015):88-99.