Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity
	Deng, Jiaoyu 5; Bi, Lijun 1,2,9; Zhou, Lin 6; Guo, Shu-juan 3,4; Fleming, Joy 1,2; Jiang, He-wei 3,4; Zhou, Ying 1,2; Gu, Jia 5; Zhong, Qiu 6; Wang, Zong-xiu 3,4
刊名	CELL REPORTS
	2014-12-24
卷号	9 期号:6 页码:2317-2329
ISSN号	2211-1247
通讯作者	Tao, SC (reprint author), Chinese Acad Sci, Inst Biophys, Key Lab Non Coding RNA, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China.
英文摘要	Poor understanding of the basic biology of Mycobacterium tuberculosis (MTB), the etiological agent of tuberculosis, hampers development of much-needed drugs, vaccines, and diagnostic tests. Better experimental tools are needed to expedite investigations of this pathogen at the systems level. Here, we present a functional MTB proteome microarray covering most of the proteome and an ORFome library. We demonstrate the broad applicability of the microarray by investigating global protein-protein interactions, small-molecule-proteinbinding, and serum biomarker discovery, identifying 59 PknG-interacting proteins, 30 bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) binding proteins, and 14 MTB proteins that together differentiate between tuberculosis (TB) patients with active disease and recovered individuals. Results suggest that the MTB rhamnose pathway is likely regulated by both the serine/threonine kinase PknG and c-di-GMP. This resource has the potential to generate a greater understanding of key biological processes in the pathogenesis of tuberculosis, possibly leading to more effective therapies for the treatment of this ancient disease.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	CYCLIC DI-GMP ; CELL-WALL ; KINASE-G ; REVEALS ; ASSAYS ; GENES ; PHOSPHORYLATION ; NORMALIZATION ; METABOLISM ; INHIBITION
收录类别	SCI
语种	英语
WOS记录号	WOS:000346852400028
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23995]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Biophys, Key Lab Non Coding RNA, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China 3.Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Minist Educ, Shanghai Ctr Syst Biomed,Key Lab Syst Biomed, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 6.Ctr TB Control Guangdong Prov, Guangzhou 510630, Guangdong, Peoples R China 7.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430071, Peoples R China 8.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China 9.TB Healthcare Biotechnol Co Ltd, Foshan 528000, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Deng, Jiaoyu,Bi, Lijun,Zhou, Lin,et al. Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity[J]. CELL REPORTS,2014,9(6):2317-2329.
APA	Deng, Jiaoyu.,Bi, Lijun.,Zhou, Lin.,Guo, Shu-juan.,Fleming, Joy.,...&Zhang, Xian-En.(2014).Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity.CELL REPORTS,9(6),2317-2329.
MLA	Deng, Jiaoyu,et al."Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity".CELL REPORTS 9.6(2014):2317-2329.