Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila | |
Chen, Gangfu1; Liu, Yang1,2,3; Jiang, Jun1,2,3; Jiang, Weidan1,2,3; Kuang, Shengyao1,2,3; Tang, Ling1,4; Tang, Wuneng1,4; Zhang, Yong-An5; Zhou, Xiaoqiu1,2,3; Feng, Lin1,2,3 | |
刊名 | FISH & SHELLFISH IMMUNOLOGY |
2015-05-01 | |
卷号 | 44期号:1页码:195-202 |
关键词 | Arginine Cyprinus carpio var. Jian Disease resistance Immune response |
ISSN号 | 1050-4648 |
通讯作者 | Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Peoples R China. |
英文摘要 | This study was conducted to test the hypothesis that elevated dietary arginine enhances immunity of juvenile Jian carp (Cyprinus carpio var. Jian). Fish were fed graded levels of dietary arginine for 9 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. Head kidney and spleen weights, as well as erythrocyte and leukocyte counts were significantly influenced by dietary arginine levels. A similar trend was also observed for hemagglutination titre, serum lysozyme activity, IgM concentration, C3 and C4 content. The highest survival rates following A. hydrophila infection were obtained in fish fed the diets containing arginine at 16.1-21.9 g/kg diet. Phagocytic activity of leukocytes was significantly enhanced by dietary arginine supplementation. In contrast, acid phosphatase activity significantly decreased with dietary arginine levels. Dietary arginine levels did not have a significant effect on the total iron-binding capacity. Gene expression of TNF-alpha and TGF-beta in head kidney significantly increased with dietary arginine levels up to 21.9 g/kg diet, and decreased thereafter. Fish fed the basal diet exhibited the highest IL-10 mRNA expression level. Gene expression of IL-1 beta and TOR increased with dietary arginine addition, reaching a plateau at 18.5 and 21.9 g arginine/kg diet, respectively. In spleen, higher IL-1 beta and TNF-alpha gene expressions were obtained in fish fed the diets containing 24.5 g arginine/kg diet than in fish fed the other dietary treatments. TGF-beta mRNA expression levels were significantly lower in fish fed the diets containing <= 21.9 g arginine/kg diet. IL-10 and TOR mRNA expression levels were lower in fish fed 16.1 g arginine/kg diet, while 4E-BP mRNA expression levels increased with dietary arginine levels up to 12.7 g/kg diet and decreased thereafter. Our results indicate that arginine has beneficial effects on regulating mRNA expression of inflammatory cytokines, as well as TOR and 4E-BP and improving humoral and cellular immunity, therefore enhancing disease resistance of fish. (C) 2015 Elsevier Ltd. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
关键词[WOS] | CATFISH ICTALURUS-PUNCTATUS ; NITRIC-OXIDE PRODUCTION ; CHANNEL CATFISH ; CYPRINUS-CARPIO ; RAINBOW-TROUT ; GROWTH-PERFORMANCE ; OXIDATIVE STRESS ; JUVENILE JIAN ; ASCORBIC-ACID ; GRADED-LEVELS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000353743900023 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/23979] |
专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Peoples R China 2.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Yaan 625014, Peoples R China 3.Sichuan Agr Univ, Sichuan Univ Key Lab, Fish Nutr & Safety Prod, Yaan 625014, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Gangfu,Liu, Yang,Jiang, Jun,et al. Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(1):195-202. |
APA | Chen, Gangfu.,Liu, Yang.,Jiang, Jun.,Jiang, Weidan.,Kuang, Shengyao.,...&Feng, Lin.(2015).Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,44(1),195-202. |
MLA | Chen, Gangfu,et al."Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 44.1(2015):195-202. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论