Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4
	Li, Shun 1; Guo, Xia 1,2; Lu, Long-Feng 1,2; Lu, Xiao-Bing 1,2; Wu, Nan 1; Zhang, Yong-An 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2015-07-01
卷号	51 期号:1 页码:65-73
关键词	IRF4 Interferon regulatory factor STAT6 c-Rel Zebrafish SVCV
ISSN号	0145-305X
通讯作者	Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
英文摘要	Interferon regulatory factor 4 (IRF4) plays pivotal roles in both innate and adaptive immune responses in mammals. In fish, there are two homologues of irf4, irf4a and irf4b. In this study, we examined the regulatory patterns of zebrafish irf4a and irf4b by STAT6 and c-Rel. Firstly, expression of irf4a and irf4b was monitored in several tissues at mRNA level. By infection with SVCV, irf4a and irf4b were upregulated in both kidney and spleen, and were immediately induced by treatment with poly I:C in ZF4 cells. Moreover, the activation of irf4a promoter was regulated by overexpression of stat6 and c-rel in a cooperation manner, which could be inhibited by mutation of the putative binding sites of STAT6 and c-Rel in irf4a promoter region. However, irf4b promoter was activated slightly only by STAT6 but not c-Rel. Furthermore, overexpression of irf4a inhibited the activation of its own promoter under induction of STAT6 and c-Rel, which was the result of that IRF4a bound to STAT6 and c-Rel directly. In addition, cellular location analysis showed that IRF4a was located only in nuclear region. These data indicate that fish irf4a can also be upregulated by STAT6 and c-Rel. (C) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Zoology
研究领域[WOS]	Immunology ; Zoology
关键词[WOS]	B-CELL DEVELOPMENT ; NF-KAPPA-B ; TRANSCRIPTION FACTOR ; INTERLEUKIN-4-INDUCED TRANSCRIPTION ; FACTOR FAMILY ; MATURE B ; EXPRESSION ; ACTIVATION ; VIRUSES ; DIFFERENTIATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000357906800008
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23967]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Shun,Guo, Xia,Lu, Long-Feng,et al. Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):65-73.
APA	Li, Shun,Guo, Xia,Lu, Long-Feng,Lu, Xiao-Bing,Wu, Nan,&Zhang, Yong-An.(2015).Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),65-73.
MLA	Li, Shun,et al."Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):65-73.