A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring

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	A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring
	Zhao, Sujuan 1,2; Li, Guangyu 3; Chen, Jun 1
刊名	JOURNAL OF PROTEOMICS
	2015-01-30
卷号	114 页码:197-213
关键词	Neurotoxicity MCLR Proteomics Offspring Generation transfer
ISSN号	1874-3919
通讯作者	Li, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
英文摘要	Recent studies showed that microcystins (MCs) can be transferred to offspring from their adults and exert notable neurotoxicity, but the exact mechanism is little known. In order to better understand cellular responses in brain tissues disrupted by prenatal transfer of MCs, this work mainly focuses on brain impairments of rat offspring. Pregnant SD rats were infused exposed to microcystin-LR (MCLR) at 10 mu g/kg body weight (BW)/day or saline solution from gestational day 8 (GD8) to postnatal day 15 (PD15) of lactation. MCLR accumulation, the levels of malondialdehyde (MDA) and acetylcholine esterase (AChE) activity were detected. The results showed that MCLR enhanced toxin accumulation and MDA, but decreased GSH and the level of AChE activity in the brains of rat offspring. MCLR also caused changes to cerebrum ultrastructure, showing a sparse structure, distention of endoplasmic reticulum and swelling mitochondria. To explore the exact mechanisms, we used a proteomic analysis to identify global brain protein profiles. The proteomic results revealed that MCLR remarkably altered the abundance of 49 proteins that were involved in neurodevelopment, oxidative phosphorylation, cytoskeleton, metabolism, protein folding and degradation. Our results indicated that MCLR exerts neurotoxicity mainly by generating oxidative stress and endoplasmic reliculum stress.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	OXIDATIVE STRESS GENERATION ; INDUCED APOPTOSIS ; ALZHEIMERS-DISEASE ; DANIO-RERIO ; PROTEIN EXPRESSION ; ORYZIAS-LATIPES ; MITOCHONDRIAL ; TOXICITY ; BRAIN ; RR
收录类别	SCI
语种	英语
WOS记录号	WOS:000348949100016
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23872]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2.Anhui Med Univ, Sch Publ Hlth, Hefei 230032, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Sujuan,Li, Guangyu,Chen, Jun. A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring[J]. JOURNAL OF PROTEOMICS,2015,114:197-213.
APA	Zhao, Sujuan,Li, Guangyu,&Chen, Jun.(2015).A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring.JOURNAL OF PROTEOMICS,114,197-213.
MLA	Zhao, Sujuan,et al."A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring".JOURNAL OF PROTEOMICS 114(2015):197-213.