Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01 | |
Zhang, Guoqiang1; Han, Lirong1; Zhang, Guifeng2; Zhang, Xing1; Feng, Juntao1 | |
刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2015-07-01 | |
卷号 | 78期号:JUL页码:195-201 |
关键词 | Streptomyces sp ZX01 Antiviral activity Tobacco mosaic virus |
ISSN号 | 0141-8130 |
英文摘要 | A novel glycoprotein GP-1 with antiviral activity against plant virus was isolated from the fermentation broth of the actinomycete Streptomyces sp. ZX01. MALDI-TOF-MS proved that molecular weight of GP-1 approximately was 8.5 kDa. GP-1 was a heat-sensitive glycoprotein with decreasing antiviral activity after treated from 80 degrees C to 100 degrees C for 30 min. GP-1 contained 40.23% carbohydrate with N-linked and O-linked glycan. FT-IR and NMR spectra proved that GP-1 contained protein and carbohydrate portions with alpha-D-(1,6)-glucose residues. Circular dichroism revealed that GP-1 was a glycoprotein with a large unordered content. Moreover, protein sequencing was predicted by using MALDI-TOF-MS and Mascot search. These results suggested that glycoprotein GP-1 could be used as a novel natural antiviral agent in agricultural industry. (C) 2015 Elsevier B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biochemistry & Molecular Biology |
研究领域[WOS] | Biochemistry & Molecular Biology |
关键词[WOS] | MYCOBACTERIUM-TUBERCULOSIS ; LIVIDANS 66 ; STRUCTURAL ELUCIDATION ; CULTURED MYCELIA ; BINDING-PROTEIN ; GLYCOSYLATION ; HYDROXYPROLINE ; BIOSYNTHESIS ; IMPROVEMENT ; PATHOGENS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000356195400026 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/19308] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Northwest A&F Univ, Res & Dev Ctr Biorat Pesticide, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Guoqiang,Han, Lirong,Zhang, Guifeng,et al. Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,78(JUL):195-201. |
APA | Zhang, Guoqiang,Han, Lirong,Zhang, Guifeng,Zhang, Xing,&Feng, Juntao.(2015).Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,78(JUL),195-201. |
MLA | Zhang, Guoqiang,et al."Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 78.JUL(2015):195-201. |
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