Molecularly imprinted solid-phase extraction for the selective determination of 17 beta-estradiol in fishery samples with high performance liquid chromatography

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Molecularly imprinted solid-phase extraction for the selective determination of 17 beta-estradiol in fishery samples with high performance liquid chromatography
	Jiang, Tianhe; Zhao, Lixia; Chu, Baolin; Feng, Qinzhong; Yan, Wei; Lin, Jin-Ming
刊名	TALANTA
	2009-04-30
卷号	78 期号:2 页码:442-447
关键词	17 beta-Estradiol Molecularly imprinted polymer Solid-phase extraction High performance liquid chromatography
中文摘要	A molecularly imprinted polymer (MIP) has been synthesized by a thermo-polymerization method using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, acetonitrile as porogenic solvent, and 17 beta-estradiol as template. The MIP showed obvious affinity for 17 beta-estradiol in acetonitrile solution, which was confirmed by absorption experiments. After optimization of molecularly imprinted solid-phase extraction (MISPE) conditions, three structurally related estrogenic compounds (17 beta-estradiol, estriol. and diethylstilbestrol) were used to evaluate the selectivity of the MIP cartridges. The MIP cartridges exhibited highly selectivity for E(2), the recoveries were 84.8 +/- 6.53% for MIPs and 19.1 +/- 1.93% for non-imprinted polymer (NIP) cartridges. The detection and quantification limits correspond to 0.023 and 0.076 mg L(-1). Furthermore, the MISPE methods were used to selectively extract E(2) from fish and prawn tissue prior to HPLC analysis. This MISPE-HPLC procedure could eliminate all matrix interference simultaneously and had good recoveries (78.3-84.5%). (c) 2008 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000264043400017
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21539]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Jiang, Tianhe,Zhao, Lixia,Chu, Baolin,et al. Molecularly imprinted solid-phase extraction for the selective determination of 17 beta-estradiol in fishery samples with high performance liquid chromatography[J]. TALANTA,2009,78(2):442-447.
APA	Jiang, Tianhe,Zhao, Lixia,Chu, Baolin,Feng, Qinzhong,Yan, Wei,&Lin, Jin-Ming.(2009).Molecularly imprinted solid-phase extraction for the selective determination of 17 beta-estradiol in fishery samples with high performance liquid chromatography.TALANTA,78(2),442-447.
MLA	Jiang, Tianhe,et al."Molecularly imprinted solid-phase extraction for the selective determination of 17 beta-estradiol in fishery samples with high performance liquid chromatography".TALANTA 78.2(2009):442-447.