Enzymatic digestion and chromatographic analysis of arsenic species released from proteins

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Enzymatic digestion and chromatographic analysis of arsenic species released from proteins
	Lu, Meiling ; Wang, Hailin ; Li, Xing-Fang ; Lu, Xiufen ; Le, X. Chris
刊名	JOURNAL OF CHROMATOGRAPHY A
	2009-05-01
卷号	1216 期号:18 页码:3985-3991
关键词	Reactive arsenic metabolites Protein-arsenic complex Speciation Protease digestion Protein-bound arsenic species Mass spectrometry
中文摘要	A method combining gel filtration chromatography (GFC), protease digestion, and ion pair chromatography with inductively coupled plasma mass spectrometry detection was developed for the determination of arsenic species bound to proteins. The method was first established by examining the interactions of two model proteins, metallothionein (MT) and hemoglobin, with three reactive trivalent arsenic species. It was then successfully applied to the speciation of arsenic in red blood cells of rats. Inorganic arsenite (iAs(III)), monomethylarsonous acid (MMA(III)), and dimethylarsinous acid (DMA(III)) were efficiently released from the proteins by protease digestion at pH 8.0, with the recovery ranging from 93% to 106%. There was no oxidation of iAs(III) or MMA(III) during the protease digestion process. Up to 61% DMA(III) (the least stable arsenic species) was unchanged, and the rest was oxidized to the pentavalent dimethylarsinic acid (DMA(V)). The arsenic species in the red blood cells of control rats was present as DMA(III) complex with hemoglobin. The method enabling the determination of the specific arsenic species that bind to cellular proteins is potentially useful for studying arsenic distribution, metabolism, and toxicity. (C) 2009 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000265699900041
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21500]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Lu, Meiling,Wang, Hailin,Li, Xing-Fang,et al. Enzymatic digestion and chromatographic analysis of arsenic species released from proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(18):3985-3991.
APA	Lu, Meiling,Wang, Hailin,Li, Xing-Fang,Lu, Xiufen,&Le, X. Chris.(2009).Enzymatic digestion and chromatographic analysis of arsenic species released from proteins.JOURNAL OF CHROMATOGRAPHY A,1216(18),3985-3991.
MLA	Lu, Meiling,et al."Enzymatic digestion and chromatographic analysis of arsenic species released from proteins".JOURNAL OF CHROMATOGRAPHY A 1216.18(2009):3985-3991.