Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

	Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase
	Zheng, YG; Li, Y; Chen, JF; Jiang, WH; Zhao, GP; Wang, ED
刊名	BIOTECHNOLOGY LETTERS
	2001
卷号	23 期号:21 页码:1781-1787
关键词	cloramphenicol glutaryl 7-amino-cephalosporanic acid acylase kanamycin secretory expression
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g(-)1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.
学科主题	Biotechnology & Applied Microbiology
类目[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	ESCHERICHIA-COLI ; PENICILLIN ACYLASE ; CEPHALOSPORIN-C ; PSEUDOMONAS ; CLONING ; GENE ; PURIFICATION ; MUTANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000171801000009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2714]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zheng, YG,Li, Y,Chen, JF,et al. Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase[J]. BIOTECHNOLOGY LETTERS,2001,23(21):1781-1787.
APA	Zheng, YG,Li, Y,Chen, JF,Jiang, WH,Zhao, GP,&Wang, ED.(2001).Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase.BIOTECHNOLOGY LETTERS,23(21),1781-1787.
MLA	Zheng, YG,et al."Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase".BIOTECHNOLOGY LETTERS 23.21(2001):1781-1787.