DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes

	DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes
	Qiu, ZL; Wei, Y; Chen, N; Jiang, MR; Wu, JR; Liao, K
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2001
卷号	276 期号:15 页码:11988-11995
通讯作者	Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Upon differentiation induction of 3T3-L1 preadipocytes by a hormone mixture containing 1-isobutyl-8-methylxanthine, dexamethasone, and insulin, the preadipocytes undergo similar to2 rounds of mitotic clonal expansion, which just; precedes the adipogenic gene expression program and has been thought to be an essential early step for differentiation initiation. By inducing 3T3-L1 preadipocytes with each individual hormone, it was determined that the mitotic clonal expansion was induced only by insulin and not by 1-isobutyl-3-methylxanthine or dexamethasone. Cell number counting an fluorescence-activated cell-sorting analysis indicated that a significant fraction of 3T3-L1 preadipocytes differentiated into adipocytes without mitotic clonal expansion when induced with the combination of 1-isobutyl-3-methylxanthine and dexamethasone. Furthermore, when normally induced 3T3-L1 preadipocytes were treated with PD98059 tan inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase 1) to block the activation of extracellular signal-regulated kinase (Erk) 1 and Erk2, the mitotic clonal expansion was blocked, but adipocyte differentiation was not affected. These observations were confirmed by bromodeoxyuridine labeling. The differentiated adipocytes induced with 1-isobutyl-3-methylxanthine and dexamethasone or standard hormone mixture plus PD98059 were not labeled by bromodeoxyuridine. Thus, it is evident that 3T3-L1 preadipocytes could differentiate into adipocytes without DNA synthesis and mitotic clonal expansion. Our results also suggested that activation of Erk1 and Erk2 is essential to but not sufficient for induction of mitotic clonal expansion.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	GROWTH-FACTOR-I ; INSULIN ; KINASE ; CELLS ; INHIBITION ; INDUCTION ; TURNOVER
收录类别	SCI
语种	英语
WOS记录号	WOS:000168081800074
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2710]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qiu, ZL,Wei, Y,Chen, N,et al. DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2001,276(15):11988-11995.
APA	Qiu, ZL,Wei, Y,Chen, N,Jiang, MR,Wu, JR,&Liao, K.(2001).DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes.JOURNAL OF BIOLOGICAL CHEMISTRY,276(15),11988-11995.
MLA	Qiu, ZL,et al."DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes".JOURNAL OF BIOLOGICAL CHEMISTRY 276.15(2001):11988-11995.