Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes

	Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes
	Feng, Y; Kong, YY; Wang, Y; Qi, GR
刊名	JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
	2001
卷号	16 期号:10 页码:1125-1130
关键词	antiviral core protein DNA replication hammerhead ribozyme hepatitis B virus
通讯作者	Qi, GR (reprint author), Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China.,
英文摘要	Background: Chronic hepatitis B virus (HBV) infection is frequently associated with cirrhosis and hepatocellular carcinoma, and so has become a major worldwide health problem. Hammerhead ribozymes have recently gained some attention as potential tools to inhibit viral infection, for which there are no general effective therapies available. Methods: A hammerhead ribozyme, RzC, was designed to target the sequence encoding the tail region of the HBV core protein. The activities of the ribozyme were analyzed in vitro and in human hepatoma (HepG2) cells. Results: In vitro, RzC cleaves HBV-RNA at its target site up to 30%, while the disabled ribozyme, dRzC, which has a one-base mutation in the catalytic site, did not cleave the target RNA at all. When the ribozymes were cotransfected into HepG2 cells with the HBV genome-containing plasmid, p3.6II, the inhibition of HBV replication by RzC was greater than that by dRzC, indicating that the active catalytic domain of the hammerhead ribozyme could increase the extent of antisense-mediated inhibition. In addition, there was a gradient of effectiveness in which the greater the amount of released ribozyme, the greater the reduction in HBV progeny DNA. Conclusions: These results suggest the possibility of hammerhead ribozyme-mediated gene therapy for the treatment of HBV infections. (C) 2001 Blackwell Science Asia Pty Ltd.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	CORE PROTEIN ; CLEAVAGE ; PHOSPHORYLATION ; SIGNAL ; CELLS ; RNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000179450900009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2706]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Feng, Y,Kong, YY,Wang, Y,et al. Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2001,16(10):1125-1130.
APA	Feng, Y,Kong, YY,Wang, Y,&Qi, GR.(2001).Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes.JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,16(10),1125-1130.
MLA	Feng, Y,et al."Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes".JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 16.10(2001):1125-1130.