Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle

	Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle
	Ruan, KC; Xu, CH; Yu, Y; Li, J; Lange, R; Bec, N; Balny, C
刊名	EUROPEAN JOURNAL OF BIOCHEMISTRY
	2001
卷号	268 期号:9 页码:2742-2750
关键词	conformational changes hydrostatic pressure spinach particle protein denaturation
通讯作者	Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The 33-kDa protein isolated from the spinach photosystem II particle is an ideal model to explore high-pressure protein-unfolding. The protein has a very low free energy as previously reported by chemical unfolding studies, suggesting that it must be easy to modulate its unfolding transition by rather mild pressure. Moreover, the protein molecule consists of only one tryptophan residue (Trp241) and eight tyrosine residues, which can be conveniently used to probe the protein conformation and structural changes under pressure using either fluorescence spectroscopy or fourth derivative UV absorbance spectroscopy. The different experimental methods used in the present study indicate that at 20 degreesC and pH 6, the 33-kDa protein shows a reversible two-state unfolding transition from atmospheric pressure to about 180 MPa. This value is much lower than those found for the unfolding of most proteins studied so far. The unfolding transition induces a large red shift of the maximum fluorescence emission of 34 nm (from 316 nm to 350 nm). The change in standard free energy (DeltaG(o)) and in volume (DeltaV) for the transition at pH 6.0 and 20 degreesC are -14.6 kJ.mol(-1) and -120 mL.mol(-1), respectively, in which the DeltaG(o) value is consistent with that obtained by chemical denaturation. We found that pressure-induced protein unfolding is promoted by elevated temperatures, which seem largely attributed to the decrease in the absolute value of DeltaG(o) (only a minor variation was observed for the DeltaV value). However, the promotion of the unfolding by alkaline pH seems mainly related to the increase in DeltaV without any significant changes in DeltaG(o). It was also found that NaCl significantly protects the protein from pressure-induced unfolding. In the presence of 1 m NaCl, the pressure needed to induce the half-unfold of the protein is shifted to a higher value (shift of 75 MPa) in comparison with that observed without NaCl. Interestingly, in the presence of NaCl, the value of DeltaV is significantly reduced whilst that of DeltaG(o) remains as before. The unfolding-refolding kinetics of the protein has also been studied by pressure-jump, in which it was revealed that both reactions are a two-state transition process with a relatively slow relaxation time of about 10(2) s.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	PHOTOSYNTHETIC OXYGEN-EVOLUTION ; DERIVATIVE UV-SPECTROSCOPY ; HIGH HYDROSTATIC-PRESSURE ; CONFORMATIONAL DRIFT ; RIBONUCLEASE-A ; DEHYDROGENASE ; DENATURATION ; CHYMOTRYPSINOGEN ; CRYOINACTIVATION ; RECONSTITUTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000168560100026
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2703]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ruan, KC,Xu, CH,Yu, Y,et al. Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY,2001,268(9):2742-2750.
APA	Ruan, KC.,Xu, CH.,Yu, Y.,Li, J.,Lange, R.,...&Balny, C.(2001).Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle.EUROPEAN JOURNAL OF BIOCHEMISTRY,268(9),2742-2750.
MLA	Ruan, KC,et al."Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle".EUROPEAN JOURNAL OF BIOCHEMISTRY 268.9(2001):2742-2750.