High IFN-alpha expression is associated with the induction of experimental autoimmune uveitis (EAU) in Fischer 344 rat

	High IFN-alpha expression is associated with the induction of experimental autoimmune uveitis (EAU) in Fischer 344 rat
	Hu, YJ; Zang, L; Wu, YD; Sun, B
刊名	CELL RESEARCH
	2001
卷号	11 期号:4 页码:293-300
关键词	experimental autoimmune uveitis (EAU) fischer 344 rat IFN-alpha pertussis toxin(PTX)
通讯作者	Sun, B (reprint author), Chinese Acad Sci, Lab Mol Immunol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,
英文摘要	Th1-response plays a crucial role in determining pathogenesis of organ-specific autoimmune diseases. It is believed that both IL-12 and INF-alpha axe initiators to regulate Th1- response. In our experimental autoimmune uveitis (EAU) model, both Lewis and Fischer 344 rats share the same MHC class II molecules, while Lewis rat is EAU susceptible and Fischer 344 rat is EAU resistant. However, under the same condition of immunization, if pertussis toxin (PTX) was injected intraperitoneally as an additional adjuvant, Fischer 344 rat can develop EAU. In this study we investigate which mechanisms axe involved in the induction of EAU in CFA+R16+PTX-treated (CRP-treated) Fischer 344 rats. In vivo and in vitro data demonstrated that Th1-cytokine, IFN-gamma mRNA expression was significantly increased in disease target tissue-eyes and in draining lymph node cells of CRP-treated Fischer 344 rat. When IL-12 and IFN-alpha mRNA expression were compared in the experimental groups, only IFN-alpha mRNA expression was associated with EAU development. To distinguish the sources of IFN-alpha producing cells, it was observed that IFN-alpha expression was mainly produced by macrophages. It was further confirmed that normal macrophage from Fischer 344 rat was able to produce significant IFN-alpha in the presence of PTX. The data strongly suggested that IFN-alpha might be involved in initiating Th1-cell differentiation and in turn contribute to the induction of EAU. High IFN-alpha expression induced by PTX may represent a novel pathway to initiate Th1 response in Fischer 344 rat.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	INTERFERON-GAMMA PRODUCTION ; GENETIC SUSCEPTIBILITY ; T-CELLS ; MICE ; ACTS ; TH1
收录类别	SCI
语种	英语
WOS记录号	WOS:000173058800007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2640]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, YJ,Zang, L,Wu, YD,et al. High IFN-alpha expression is associated with the induction of experimental autoimmune uveitis (EAU) in Fischer 344 rat[J]. CELL RESEARCH,2001,11(4):293-300.
APA	Hu, YJ,Zang, L,Wu, YD,&Sun, B.(2001).High IFN-alpha expression is associated with the induction of experimental autoimmune uveitis (EAU) in Fischer 344 rat.CELL RESEARCH,11(4),293-300.
MLA	Hu, YJ,et al."High IFN-alpha expression is associated with the induction of experimental autoimmune uveitis (EAU) in Fischer 344 rat".CELL RESEARCH 11.4(2001):293-300.