Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization

	Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization
	Du, ZW; Cong, HC; Yao, Z
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2001
卷号	282 期号:3 页码:701-706
关键词	Oct-4 downstream gene suppression-subtractive hybridization embryonic stem cell
通讯作者	Du, ZW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,
英文摘要	As a step toward understanding how toti/pluripotence is maintained by Oct-4, we have first constructed a cell model with differentially expressed Oct-4 in embryonic stem cells, and then used suppression-subtractive hybridization (SSH) method to identify the downstream genes of Oct-4. Among the 384 clones we screened, 40 clones were detected as differentially expressed genes with colony hybridization, and 13 clones were confirmed as the putative downstream genes of Oct-4 by Northern blot analysis. Sequencing showed 12 different genes, 8 known genes (Oct-4, Rex-1, Sox-2, Creatine kinase B, Makorin 1, Importin beta, Histone H2A.Z, Ribosomal protein S7) and 4 new genes. Except Oct-4 and Rex-1, the other genes have not been reported to be regulated by Oct-4, These results showed that SSH provides a very efficient means to identify the downstream genes of transcription factor. Some known genes identified may provide new insight of the function of Oct-4 in stem cells. (C) 2001 Academic Press.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	EMBRYONIC STEM-CELLS ; TRANSCRIPTION FACTOR ; EXPRESSION ; DIFFERENTIATION ; SOX-2 ; PROMOTER ; ENHANCER ; BINDING ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000168092000008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2632]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Du, ZW,Cong, HC,Yao, Z. Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,282(3):701-706.
APA	Du, ZW,Cong, HC,&Yao, Z.(2001).Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,282(3),701-706.
MLA	Du, ZW,et al."Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 282.3(2001):701-706.