Cystatin 11: A new member of the cystatin type 2 family

	Cystatin 11: A new member of the cystatin type 2 family
	Hamil, KG; Liu, Q; Sivashanmugam, P; Yenugu, S; Soundararajan, R; Grossman, G; Richardson, RT; Zhang, YL; O'Rand, MG; Petrusz, P
刊名	ENDOCRINOLOGY
	2002
卷号	143 期号:7 页码:2787-2796
通讯作者	Hall, SH (reprint author), Univ N Carolina, Reprod Biol Lab, Sch Med, CB 7500, Chapel Hill, NC 27599 USA.,
英文摘要	Cystatin (CST)11, a novel member of the CST type 2 family of cysteine protease inhibitors, was identified in Macaca mulatta epididymis by subtractive hybridization cloning. The human CST11 gene on chromosome 20p11.2 is located near three other CST genes expressed predominantly in the male reproductive tract. The CST11 gene spans three exons, a structure similar to that of other CST family 2 genes. An exon 2-deleted alternative transcript (CST11Delta2) was also identified. CST11 mRNA is expressed only in the epididymis as judged by Northern blot hybridization and is androgen regulated. The protein is most abundant in the initial segment, but is detected throughout the epididymis and on ejaculated human sperm. The calculated tertiary structure of CST11 reveals that the three regions corresponding to the protease inhibitory wedge of CST3 are similarly juxtaposed in CST11, consistent with protease inhibitor function. Intact and exon 2-deleted CST11 recombinant proteins were tested for antibacterial activity. After a 2-h incubation of Escherichia coli with 50 mug/ml recombinant CST11 or CST11Delta2, bacterial colony-forming units were reduced to 30% of control, indicating that both forms have antimicrobial activity.
学科主题	Endocrinology & Metabolism
类目[WOS]	Endocrinology & Metabolism
关键词[WOS]	3-DIMENSIONAL SOLUTION STRUCTURE ; CYSTEINE PROTEINASE-INHIBITOR ; RAY CRYSTAL-STRUCTURE ; HERPES-SIMPLEX VIRUS ; CATHEPSIN-L ; PROTEASE INHIBITORS ; SERTOLI CELLS ; MESSENGER-RNA ; CRES GENE ; IN-VITRO
收录类别	SCI
语种	英语
WOS记录号	WOS:000176550400041
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2524]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hamil, KG,Liu, Q,Sivashanmugam, P,et al. Cystatin 11: A new member of the cystatin type 2 family[J]. ENDOCRINOLOGY,2002,143(7):2787-2796.
APA	Hamil, KG.,Liu, Q.,Sivashanmugam, P.,Yenugu, S.,Soundararajan, R.,...&Hall, SH.(2002).Cystatin 11: A new member of the cystatin type 2 family.ENDOCRINOLOGY,143(7),2787-2796.
MLA	Hamil, KG,et al."Cystatin 11: A new member of the cystatin type 2 family".ENDOCRINOLOGY 143.7(2002):2787-2796.