Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia

	Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia
	Wang, JH; Yao, MZ; Gu, JF; Sun, LY; Shen, YF; Liu, XY
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	290 期号:5 页码:1454-1461
关键词	heat shock transcription factor 1 heat shock protein 70 dominant-negative mutant hyperthermia apoptosis gene therapy retrovirus breast cancer
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Heat shock protein 70 (HSP70), an antiapoptotic chaperon protein, is highly expressed in human breast tumors and renders them resistant to such therapy as hyperthermia. In the present study, we inhibited the expression of HSP70 by blocking the heat shock transcription factor 1 (HSF1) function with its dominant-negative mutant (mHSF1) in Bcap37 cells, a thermo-tolerant breast cancer cell line. Here we report that retrovirus-mediated transfer of mHSF1 led to massive cell death of Bcap37 after hyperthermia. mHSF1 sensitized Bcap37 cells to hyperthermia by promoting apoptosis induced by heat shock. We also examined the efficacy of mHSF1 gene therapy in the nude mouse. mHSF1 transfection led to diminution of tumor growth with hyperthermia therapy. Thus, disrupting HSF1 in combination with hyperthermia may open new possibilities for treatment of cancers that have acquired resistance to heat treatment. (C) 2002 Elsevier Scienre (USA).
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	HEAT-SHOCK-PROTEIN ; BREAST-CANCER ; TRANSCRIPTION FACTORS ; TRANSGENIC MICE ; HSP70 ; APOPTOSIS ; OVEREXPRESSION ; RESISTANCE ; STRESS ; CYTOTOXICITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000173783900015
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2513]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, JH,Yao, MZ,Gu, JF,et al. Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,290(5):1454-1461.
APA	Wang, JH,Yao, MZ,Gu, JF,Sun, LY,Shen, YF,&Liu, XY.(2002).Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,290(5),1454-1461.
MLA	Wang, JH,et al."Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 290.5(2002):1454-1461.