Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells

	Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells
	Liu, Z; Yang, GZ; Chi, CW; Wu, XF
刊名	PROTEIN AND PEPTIDE LETTERS
	2002
卷号	9 期号:5 页码:419-426
关键词	BmKITa1 E. coli baculovirus expression system Tn-5B1-4 cell
通讯作者	,
英文摘要	BmK ITal cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni2+-IDA-sepharose 6B column was toxic for insect, which indicated that it was biologically activity. Furthermore, Quantitative estimation show that the biological activity of recombinant BmK ITal from Tn cells was more powerful than from K coli.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	BUTHUS-MARTENSI KARSCH ; SELECTIVE NEUROTOXIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000177915300007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2498]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, Z,Yang, GZ,Chi, CW,et al. Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells[J]. PROTEIN AND PEPTIDE LETTERS,2002,9(5):419-426.
APA	Liu, Z,Yang, GZ,Chi, CW,&Wu, XF.(2002).Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells.PROTEIN AND PEPTIDE LETTERS,9(5),419-426.
MLA	Liu, Z,et al."Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells".PROTEIN AND PEPTIDE LETTERS 9.5(2002):419-426.