Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages

	Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages
	Wang, Y; Li, CL; Wang, XY; Zhang, JS; Chang, ZL
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	296 期号:3 页码:742-748
关键词	heat shock response IL-18 JNK AP-1 macrophages
通讯作者	Chang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,
英文摘要	Heat shock response has been implicated in cytoprotective effects from cellular damage and in the regulation of cytokine expression. We report the effect of heat shock on LPS-induced expression of IL-18, an important cytokine that has diverse immune regulatory effects on T cells, B cells, NK cells, and nonimmune cells. The augmentation of LPS-induced IL-18 mRNA and protein was significantly suppressed in murine peritoneal macrophages after 43 degreesC heat shock treatment. In addition, the JNK MAPK inhibitor SP600125 inhibited IL-18 mRNA transcription in a dose-dependent manner. To examine the possibility that the inhibition of IL-18 may be mediated through the inactivation of JNK, the activity of JNK was measured by using Western blot and kinase assays. Our data show that heat shock response decreased LPS-induced phosphorylation of JNK and its downstream substrate c-Jun. AP-1, a transcriptional factor composed of c-Jun, could regulate the expression of IL-18. Also, its DNA-binding activity was reduced by the heat shock response. These findings suggest that treatment of heat stress results in inhibition of IL-18 production in macrophages mainly through the JNK/AP-1 signaling pathway. (C) 2002 Elsevier Science (USA). All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	NF-KAPPA-B ; GENE-EXPRESSION ; IFN-GAMMA ; INTERLEUKIN-12 ; ACTIVATION ; PROTEINS ; KINASES
收录类别	SCI
语种	英语
WOS记录号	WOS:000177747800036
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2457]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, Y,Li, CL,Wang, XY,et al. Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,296(3):742-748.
APA	Wang, Y,Li, CL,Wang, XY,Zhang, JS,&Chang, ZL.(2002).Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,296(3),742-748.
MLA	Wang, Y,et al."Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 296.3(2002):742-748.