Intron requirement for AFP gene expression in Trichoderma viride

	Intron requirement for AFP gene expression in Trichoderma viride
	Xu, J; Gong, ZZ
刊名	MICROBIOLOGY-SGM
	2003
卷号	149 期号:1 页码:3093-3097
通讯作者	Gong, ZZ (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Box 16,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The 430 bp ORF of the Aspergillus giganteus antifungal protein (AFP) gene, containing two small introns, was fused between the promoter and the terminator of the Aspergillus nidulans trpC gene. The AFP gene in this vector produced detectable levels of spliced mRNA in Trichoderma viride. In contrast, in the same vector configuration, its 285 bp intronless derivative showed no accumulation of mRNA when transformed into T viride. Such expression results were confirmed at the protein level. This fact demonstrated that the introns were required for AFP gene expression in T. viride. This is thought to be a novel phenomenon found in filamentous fungi. Although the mechanism of splicing in filamentous fungi might be similar to that in other eukaryotes, little is known of how it affects expression. This study suggests that the small introns in filamentous fungal genes may not only act as intervening elements, but may also play crucial roles in gene expression by affecting mRNA accumulation. Furthermore, it may provide new evidence for intron-dependent evolution.
学科主题	Microbiology
类目[WOS]	Microbiology
关键词[WOS]	MESSENGER-RNA ; ASPERGILLUS-GIGANTEUS ; ANTIFUNGAL-PROTEIN ; DEPENDENT EVOLUTION ; SPLICE JUNCTION ; CELLS ; TRANSFORMATION ; DEHYDROGENASE ; NIDULANS ; PROMOTER
收录类别	SCI
语种	英语
WOS记录号	WOS:000186737200007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2403]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, J,Gong, ZZ. Intron requirement for AFP gene expression in Trichoderma viride[J]. MICROBIOLOGY-SGM,2003,149(1):3093-3097.
APA	Xu, J,&Gong, ZZ.(2003).Intron requirement for AFP gene expression in Trichoderma viride.MICROBIOLOGY-SGM,149(1),3093-3097.
MLA	Xu, J,et al."Intron requirement for AFP gene expression in Trichoderma viride".MICROBIOLOGY-SGM 149.1(2003):3093-3097.