Tertiary structure base pairs between D- and T psi C-loops of Escherichia coli tRNA(Leu) play important roles in both aminoacylation and editing

	Tertiary structure base pairs between D- and T psi C-loops of Escherichia coli tRNA(Leu) play important roles in both aminoacylation and editing
	Du, X; Wang, ED
刊名	NUCLEIC ACIDS RESEARCH
	2003
卷号	31 期号:11 页码:2865-2872
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	To ensure the fidelity of protein biosynthesis, aminoacyl-tRNA synthetases (aaRSs) must recognize the tRNA identity elements of their cognate tRNAs and discriminate their cognate amino acids from structurally similar ones through a proofreading (editing) reaction. For a better understanding of these processes, we investigated the role of tRNA(Leu) tertiary structure in the aminoacylation and editing reactions catalyzed by leucyl-tRNA synthetase (LeuRS). We constructed a series of Escherichia coli tRNA(Leu) mutated transcripts with alterations of the nucleotides involved in tertiary interactions. Our results revealed that any disturbance of the tertiary interaction between the tRNA(Leu) D- and TpsiC-loops affected both its aminoacylation ability and its ability to stimulate the editing reaction. Moreover, we found that the various tertiary interactions between the D- and TpsiC-loops (G18:U55, G19:C56 and U54:A58) functioned differently within the aminoacylation and editing reactions. In these two reactions, the role of base pair 19:56 was closely correlated and dependent on the hydrogen bond number. In contrast, U54:A58 was more important in aminoacylation than in editing. Taken together, our results suggest that the elbow region of tRNA formed by the tertiary interactions between the D- and TpsiC-loops affects the interactions between tRNA and aaRS effectively both in aminoacylation and in editing.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	TRANSFER-RNA-SYNTHETASE ; PHENYLALANINE TRANSFER-RNA ; CRYSTAL-STRUCTURE ; YEAST ; DISCRIMINATION ; RECOGNITION ; DETERMINANTS ; PURIFICATION ; IDENTITY ; OVERPRODUCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000183231000026
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2364]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Du, X,Wang, ED. Tertiary structure base pairs between D- and T psi C-loops of Escherichia coli tRNA(Leu) play important roles in both aminoacylation and editing[J]. NUCLEIC ACIDS RESEARCH,2003,31(11):2865-2872.
APA	Du, X,&Wang, ED.(2003).Tertiary structure base pairs between D- and T psi C-loops of Escherichia coli tRNA(Leu) play important roles in both aminoacylation and editing.NUCLEIC ACIDS RESEARCH,31(11),2865-2872.
MLA	Du, X,et al."Tertiary structure base pairs between D- and T psi C-loops of Escherichia coli tRNA(Leu) play important roles in both aminoacylation and editing".NUCLEIC ACIDS RESEARCH 31.11(2003):2865-2872.